摘要
目的:比较不同大孔树脂对淫羊藿总黄酮的吸附性能,阐明其吸附机制,优选适合纯化淫羊藿总黄酮的大孔树脂。方法:分别以淫羊藿总黄酮和5种代表性成分(朝藿定A,朝藿定B,朝藿定C,淫羊藿苷,宝霍苷Ⅰ)的吸附率、比吸附量、洗脱率、比洗脱量为指标,采用静态吸附和动态吸附相结合的方法,比较HPD100,HPD600,AB-8,X-5,D101共5种型号大孔树脂的吸附特性;利用伪一阶、伪二阶动力学模型和颗粒内扩散模型对优选树脂的吸附动力学进行研究,运用Langmuir和Freundlich等温吸附模型对优选树脂的热力学过程进行分析,从物理化学角度探究树脂的吸附机制。结果:HPD100型大孔吸附树脂对淫羊藿总黄酮的吸附和洗脱性能均较好,显著优于其他树脂;其对淫羊藿总黄酮及5种代表性黄酮的吸附动力学过程均符合伪二阶动力学模型,对淫羊藿总黄酮的吸附热力学过程符合Freundlich模型,对5种黄酮类成分之和的吸附热力学过程符合Langmuir模型;HPD100型大孔树脂对淫羊藿总黄酮的吸附过程为物理吸附占主导的放热过程,最适吸附温度25℃。结论:HPD100型大孔树脂吸附容量大、洗脱顺畅,吸附机制明确,适用于淫羊藿总黄酮的分离与纯化。
Objective: To compare the adsorption characteristics of different macroporous adsorption resins for the total flavonoids in Epimedii Folium,clarify the adsorption mechanism,and screen the optimal resin for the purification of total flavonoids in Epimedii Folium. Method: Taking the adsorption and desorption capacities of the total flavonoids in Epimedii Folium and five representative flavonoids( epimedin A,epimendin B,epimendin C,icariin,baohuoside Ⅰ) as indexes,static adsorption and dynamic adsorption experiments were conducted to compare the adsorption characteristics of five macroporous including HPD100,HPD600,AB-8,X-5 and D101. The adsorption kinetics of the selected resin was studied by using the pseudo-first-order,pseudo-secondorder kinetic models and intraparticle diffusion model,and the thermodynamic process was analyzed by using the Langmuir and Freundlich isothermal adsorption models,which explored the adsorption mechanism of resin from the perspective of physical chemistry. Result: HPD100 macroporous resin had a better adsorption and desorption properties than the others. The adsorption process of HPD100 macroporous resin for total flavonoids in Epimedii Folium and five representative flavonoids conformed to the pseudo-second-order kinetic model. The adsorption thermodynamic process of HPD100 resin for total flavonoids of Epimedii Folium conformed to the Freundlich model,and for the sum of five representative flavonoids conformed to the Langmuir model. The adsorption process of HPD100 resin for total flavonoids in Epimedii Folium was the exothermic process dominated by physical adsorption,and the optimal adsorption temperature was 25 ℃. Conclusion: HPD100 macroporous resin has large adsorption capacity,easy desorption and clear adsorption mechanism,it is suitable for isolation and purification of total flavonoids in Epimedii Folium.
作者
杨茹
刘聪燕
许婷
李瑞云
陈彦
YANG Ru;LIU Cong-yan;XU Ting;LI Rui-yun;CHEN Yan(Affiliated Hospital of Integrated Traditional Chinese and Western Medicine,Nanjing University of Chinese Medicine,Nanjing 210028,China;Multi-component of Traditional Chinese Medicine(TCM)and Microecology Research Center,Jiangsu Province Academy of TCM,Nanjing 210028,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2020年第6期113-120,共8页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家“重大新药创制”科技重大专项(2018ZX09301030-008)
江苏省科教强卫医学重点人才项目(ZDRCA2016036)。
关键词
淫羊藿
总黄酮
大孔吸附树脂
静态吸附
动态吸附
吸附动力学
吸附热力学
Epimedii Folium
total flavonoids
macroporous adsorption resin
static adsorption
dynamic adsorption
adsorption kinetics
adsorption thermodynamics
