摘要
目的研究凉粉草醇提物不同极性部位抗氧化活性及对α-葡萄糖苷酶抑制作用,并筛选最优部位。方法制备凉粉草醇提液,依次用石油醚、三氯甲烷、乙酸乙酯、水饱和正丁醇萃取得到不同极性部位,测定1,1-二苯基-2苦基肼自由基(DPPH自由基)、2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐自由基(ABTS自由基)清除能力及铁离子抗氧化能力(FRAP)值,根据清除率计算半数抑制率(IC50值),考察不同部位抗氧化能力。通过比较不同部位对α-葡萄糖苷酶抑制能力,考察不同部位对α-葡萄糖苷酶抑制作用。测定各部位多酚含量。结果凉粉草提取物不同极性部位均有抗氧化活性,其中乙酸乙酯部位抗氧化活性最强,DPPH·清除率IC50为19.13μg·mL^-1,ABTS+清除率IC50为18.20μg·mL^-1,FRAP值平均相当于Fe2+392.07μg·mL^-1。不同部位对α-葡萄糖苷酶抑制作用比较,正丁醇部位最优。结论凉粉草提取物及不同极性部位均具有一定抗氧化活性及对α-葡萄糖苷酶的抑制作用。该研究可为凉粉草开发利用提供实验依据。
Objective To study the antioxidant activity and inhibitory effect ofα-glucosidase of different polar fractions of ethanol extract from Mesona chinensis,and to selected the optimal extract.Methods The ethanol extract of Mesona chinensis was prepared,and then extracted with petroleumether,chloroform,ethyl acetate,and water saturated n-butanol to obtain different polarity parts.Determination the scavenging capacity of 1,1-Diphenyl-2-picrylhydrazyl(DPPH)free radical,2,2’-azino-bis(3-EthylBenzo-thiazoline-6-sulfonic acid)diammonium salt(ABTS)free radical and ferric ion reducing antioxidant power(FRAP)value,and the half-inhibition rate(IC50 value)was calculated based on the scavenging rate to investigate the antioxidant capacity of different parts.By comparing the inhibitory ability of different sites onα-glucosidase,to investigate different parts of the best inhibition ofα-glucosidase.The content of polyphenols in different parts were determined.Results The results showed that every polarity fraction has antioxidant function and the ethyl acetate fraction has the strongest antioxidant capacity.The IC50of DPPH·scavenging rate was 19.13μg·mL^-1,the IC50 of ABTS+scavenging rate was 18.20μg·mL^-1,and the FRAP value was equivalent to Fe2+392.07μg·mL^-1.Comparison ofα-glucosidase inhibitory effects of different fractions,the n-butanol fraction was the best.Conclusion The extract and the different polar fractions have certain antioxidant activity and inhibition ofα-glucosidase.This study can provide a theoretical basis for further research and development of Mesona chinensis Benth.
作者
宋晓娟
卢晓莹
曾唯雅
伍铭坤
庄伟权
严萍
詹若挺
SONG Xiaojuan;LU Xiaoying;ZENG Weiya;WU Mingkun;ZHUANG Weiquan;YAN Ping;ZHAN Ruoting(Center for Traditional Chinese Medicine Resources Science and Engineering,Guangzhou University of Traditional Chinese Medicine,Key Laboratory of Lingnan Traditional Chinese Medicine Resources Ministry of Education(Guangzhou University of Traditional Chinese Medicine),National Pharmaceutical Research and Technology Research Center of South China Medicine,Guangdong Provincial Department of Education Key Enhancement Platform Construction Project-Key Laboratory of Lingnan Traditional Chinese Medicine Resources,Ministry of Education,Guangzhou 510006,China)
出处
《医药导报》
CAS
北大核心
2020年第3期286-291,共6页
Herald of Medicine
基金
广东省普通高校创新团队项目——中药资源创新研究团队(2016KYTD02)
广州中医药大学2017年高水平大学建设项目(广中医研〔2017〕10号)
广东省教育厅重点提升平台建设项目——岭南中药资源教育部重点实验室(2014KTSPT016)。
关键词
凉粉草
极性部位
抗氧化活性
Α-葡萄糖苷酶
Mesona chinensis
Polar fraction
Antioxidant activities
α-glucosidase
