摘要
安丝菌素是从珍贵橙色束丝放线菌的发酵液中分离出来的苯安莎类抗生素,具有显著的抗肿瘤活性。常采用乙酸乙酯对发酵液进行分离萃取,然而其发酵液成分复杂,除菌丝体外还含有许多杂蛋白质及易溶于有机溶剂的色素,这对后续提取和精制有很大的影响,影响产品质量和回收率。本文通过对AP-3发酵液进行预处理,将AP-3发酵液调整pH至3后,用10g/L硅藻土辅助抽滤;收集滤液,添加20g/L活性炭于60℃吸附2h,然后用洗脱液乙酸乙酯对活性炭进行脱附处理,2h后达到动态洗脱平衡,对洗脱液采用中性氧化铝柱层析,以乙酸乙酯和石油醚作为洗脱剂进行梯度洗脱,最终获得AP-3纯度86.15%,经重结晶后得到AP-3纯品,经过HPLC检测AP-3纯度达95%。
Ansamitocin was a benzoan antibiotic isolated from the fermentation broth of microorganisms(Actinosynnema pretiosum) and had significant antitumor activity. The fermentation broth was often separated and extracted by ethyl acetate. However, the composition of fermentation broth was complex. In addition to mycelium, the fermentation broth also contained many heteroproteins and pigments that were easily soluble in organic solvents, which had a great impact on subsequent extraction and refining,affecting product quality and recovery. In this paper, the AP-3 fermentation broth was properly pretreated. The AP-3 fermentation broth was adjusted to pH 3, and then filtered with 10 g/L diatomaceous earth. The filtrate was collected, and 20 g/L activated carbon was added to adsorb at 60℃for 2 h. The activated carbon was desorbed with ethyl acetate as the eluent, and the dynamic elution equilibrium was reached after 2 h. The eluent was subjected to neutral alumina column chromatography, and washed with ethyl acetate and petroleum ether. The elution was carried out by gradient elution, and finally the purity of AP-3 was 86.15%. After recrystallized, AP-3 pure product was obtained, and its HPLC purity reached95%.
作者
吴兴可
祁荃
卞婷婷
刘苏煜
谷艺明
郭静
蔡志强
WU Xingke;QI Quan;BIAN Tingting;LIU Suyu;GU Yiming;GUO Jing;CAI Zhiqiang(School of Pharmaceutical Engineering&Life Science,Changzhou University,Changzhou 213164,Jiangsu,China)
出处
《化工进展》
EI
CAS
CSCD
北大核心
2020年第3期1122-1128,共7页
Chemical Industry and Engineering Progress
基金
国家自然科学基金(11275033)。
关键词
安丝菌素P-3
预处理
活性炭
吸附
分离纯化
ansamitocin P-3
pretreatment
activated carbon
adsorption
isolation/purification
