摘要
背景:聚乳酸具有良好的生物韧性和生物相容性,但生物惰性限制了聚乳酸树脂种植体植入体内后与周围组织的相互作用。因此,提高聚乳酸树脂的生物活性、改善骨结合性能的研究逐渐成为生物材料的热点问题。目的:观察聚乳酸树脂及其结合不同生物活性涂层对成骨细胞黏附、增殖及分化的影响,分析其抗菌能力。方法:3D打印聚乳酸仿生肱骨试件,并在其表面分别制备壳聚糖-α-β-GP温敏水凝胶涂层与壳聚糖纳米颗粒涂层。将MC3T3-E1细胞分别接种于3D打印聚乳酸仿生肱骨(空白组)、含壳聚糖-α-β-GP温敏水凝胶涂层3D打印聚乳酸仿生肱骨(水凝胶组)与含壳聚糖纳米颗粒涂层3D打印聚乳酸仿生肱骨(纳米颗粒组)表面,观察细胞的黏附、增殖与分化情况,检测细胞黏附斑、护骨素的基因与蛋白表达及p65、p-p65蛋白表达水平,检测细胞的白细胞介素6分泌量。将革兰阳性与革兰阴性细菌分别接种于3种试件表面,观察试件表面的细菌黏附情况。结果与结论:①接种8 h,纳米颗粒组细胞黏附数量多于水凝胶组、空白组(P<0.05),水凝胶组多于空白组(P<0.05);②纳米颗粒组、水凝胶组培养2,4,6,8 d的细胞增殖活力高于空白组(P<0.05);③培养6 d后,纳米颗粒组、水凝胶组的细胞黏附斑及护骨素基因与蛋白表达均高于空白组(P<0.05),纳米颗粒组高于水凝胶组(P<0.05);④培养8 d后,纳米颗粒组、水凝胶组的碱性磷酸酶活性与白细胞介素6分泌量高于空白组(P<0.05),纳米颗粒组高于水凝胶组(P<0.05);纳米颗粒组p-p65蛋白水平高于水凝胶组(P<0.05),水凝胶组高于空白组(P<0.05);⑤纳米颗粒组对抗革兰阳性与革兰阴性细菌的能力强于水凝胶组、空白组(P<0.05),水凝胶组强于空白组(P<0.05);⑦结果表明,3D打印聚乳酸肱骨结合-α-β-GP温敏水凝胶和壳聚糖纳米颗粒生物活性涂层均可有效促进成骨细胞的黏附、增殖及
BACKGROUND: Polylactic acid has good bio-toughness and biocompatibility, but bio-inertity limits the interaction of polylactic acid resin implants with surrounding tissues after implantation in vivo. Therefore, research on improving the biological activity of polylactic acid resin and improving bone binding properties has gradually become a hotspot of biological materials. OBJECTIVE: To observe the effects of polylactic acid resin combined with different bioactive coatings on the adhesion, proliferation and differentiation of osteoblasts, and to analyze their antibacterial ability. METHODS: The three-dimensional printed polylactic acid biomimetic tibia specimens were made, and chitosan-α-β-GP thermosensitive hydrogel coating and chitosan nanoparticle coating were prepared on the surface. MC3 T3-E1 cells were inoculated separately into three-dimensional printed polylactic acid biomimetic tibia(blank group), chitosan-α-β-GP thermosensitive hydrogel coated three-dimensional printed polylactic acid biomimetic tibia(hydrogel group) and shell-containing coating three-dimensional printed polylactic acid biomimetic tibia(nanoparticle group) surface. The cell adhesion, proliferation, and differentiation were observed. The cell adhesion plaque and osteoprotegerin gene and protein expression levels, and the expression levels of p65 and p-p65 were detected. The interleukin-6 secretion was detected. Gram-positive and Gram-negative bacteria were inoculated on the surface of the three samples, respectively, and the bacterial adhesion was observed. RESULTS AND CONCLUSION:(1) After 8 hours of inoculation, the number of adherent cells in the nanoparticle group was higher than that in the hydrogel and blank groups(P < 0.05), and the number of adherent cells hydrogel group was higher than that in the blank group(P < 0.05).(2) Cell viability in the nanoparticle and gel groups was higher than that in the blank group at 2, 4, 6 and 8 days(P < 0.05).(3) After 6 days of culture, the cell adhesion spots and the expression levels of os
作者
张忠岩
李玉波
祁同宁
常韬
Zhang Zhongyan;Li Yubo;Qi Tongning;Chang Tao(Department of Traumatic Orthopedics,Affiliated Hospital of Chengde Medical University,Chengde 067000,Hebei Province,China;Department of Traumatic Orthopedics,Chengde City Hospital,Chengde 067000,Hebei Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2020年第16期2485-2492,共8页
Chinese Journal of Tissue Engineering Research
基金
承德市科技计划自筹经费项目(201707A008)，项目负责人：张忠岩~~
关键词
3D打印
聚乳酸
仿生肱骨
壳聚糖
α-β-GP温敏水凝胶
壳聚糖纳米颗粒
细胞增殖
抗菌能力
three-dimensional printing
polylactic acid
bionic humerus
chitosan
α-β-GP temperature-sensitive hydrogel
chitosan nanoparticles
cell proliferation
anti-bacterial ability
