摘要
九层塔(Ocimum basilicum L.)为一年生唇形科药食两用植物.为研究野生九层塔植株内生细菌的多样性及生物学功能,采用LB、VM、DN和CCM4种培养基从植物不同部位中分离纯化内生细菌,利用IS-PCR插入序列指纹图谱技术进行聚类分析,通过16S rRNA基因测序分析对内生细菌进行分子鉴定及系统发育分析,进一步采用传统方法鉴定各类群代表菌株的生物学功能,并选取功能性较强的菌株进行水稻种子萌发及盆栽促生试验.结果显示,从九层塔中分离获得48株内生细菌,聚为13个类群,表现出九层塔内生菌的多样性;生物学功能测定显示所获得内生菌功能丰富:9株具有固氮功能,8株具溶磷功能,10株具有解钾能力,12株具有分泌生长素的能力,7株具有分泌铁载体功能;筛选出2个促生功能优良的菌株,分别为H1041和H4039,其发酵液能显著地促进水稻种子萌发和水稻生长.本研究表明从野生植物九层塔中分离所得的内生菌具有种群多样性和功能多样性,这些兼具不同功能的菌株具有农业应用开发的价值和意义.
Ocimum basilicum L.is an annual medicinal and edible plant of Labiatae.To investigate the biodiversity and physiological function of endophytic bacteria,the plant Ocimum basilicum L.was chosen as the experimental material.Endophytic bacteria were isolated and purified from the plant by LB,VM,DN,and CCM media.Genetic diversity of the isolates was studied by insertion sequence-based PCR(IS-PCR)finger-printing.The 16 S rRNA gene was subjected to phylogenetic analysis.Physiological and biochemical tests were used to identify the representative strains of all clusters.The biological functions of representative strains from various groups were further identified by traditional methods.The strains with strong functions were selected for further rice seed germination and pot culture.As a result,48 endophytic bacteria were isolated and purified from the Ocimum basilicum L.that were clustered into 13 groups using the IS-PCR fingerprinting method,showing the diversity of endophytic bacteria in the Ocimum basilicum L.Biological function tests showed that the endophytic bacteria had abundant functions,in which 9 strains had the function of nitrogen fixation,8 strains with solubility function,10 strains with potassium solubility,12 strains with auxin secretion,and 7 strains with siderophore secretion.Two strains,H1041 and H4039,with excellent functions were screened out.Their fermentation could significantly promote the germination of rice seeds and growth of rice.This experiment showed that the endophytic strains isolated from Ocimum basilicum L.have abundant diversity both in type and function.
作者
黄淑芬
常华瑜
顾李成
宋旭
江宝茹
谭志远
彭桂香
HUANG Shufen;CHANG Huayu;GU Licheng;SONG Xu;JIANG Baoru;TAN Zhiyuan;PENG Guixiang(College of Agriculture,South China Agricultural University,Guangzhou 510642,China;College of Natural Resources and Environment,South China Agricultural University,Guangzhou 510642,China)
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2020年第1期96-105,共10页
Chinese Journal of Applied and Environmental Biology
基金
国家自然科学基金项目(31370052)
广东省科技项目(2018A050506075,2018B020205003,2018B020206001)
广东省植物分子育种重点实验室资助。
关键词
九层塔
植物内生细菌
固氮能力
生物学功能
促生作用
Ocimum basilicum L.
endophytic bacteria
nitrogen fixation
biological function
promoting effect
