摘要
采用插入序列指纹图谱分析(IS-PCR)和16S rRNA基因全序列分析,对来源于优良牧草———糖蜜草M elinism inutifloraBeauv.内生固氮菌进行研究.结果表明,糖蜜草中分离的内生固氮菌与产脂固氮螺菌模式菌株DSM1691的插入序列指纹图谱存在着差异;来源于糖蜜草的4株菌紧密地聚在一起,与GenBank中已知产脂固氮螺菌Azospirillum lipoferum的16S rRNA基因全序列有97%的序列相似性.初步确定糖蜜草中分离的内生固氮菌为一新类群的内生固氮菌.
Endophytic diazotrophs isolated from molassess grass, Melinis minutiflora , were studied by insert sequence（IS）-PCR fingerprinting patterns and full length of 16S rRNA gene sequences. IS-PCR fingerprinting showed that different patterns appeared between isolates and the type strain DSM 1691 of Azospirillum lipoferum; 16S rRNA gene sequence analyses indicated that homology between isolates and known species Azospirillum lipoferum is 97%. These resuks suggested that isolates from molasses grass are possible a new group of endophytic diazotrophs closely related to Azospirillum lipoferum.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2005年第4期73-76,共4页
Journal of South China Agricultural University
基金
国家自然科学基金资助项目(30300001和30470002)
华南农业大学校长基金资助项目(4100-K03113)
关键词
糖蜜草
内生固氮菌
固氮螺菌
Melinis minutiflora
endophytic diazotrophs
Azospirillum lipoferum
