摘要
目的:建立琼脂糖凝胶电泳法测定白蛋白纯度。方法:采用Helana公司Epalyzer 2型全自动电泳仪及其配套的试剂盒,供试品溶液为2%,染色液浓度为0.35%,染色液体积为800μL,上样量为50μL,对153批白蛋白样品进行测定,并与醋酸纤维素薄膜法测定结果进行比较。结果:琼脂糖凝胶电泳法定量测定区间为1%~5%,最低检测浓度为0.1%;精密度考察RSD小于0.5%;本法(蛋白纯度均值96.7%)与醋酸纤维素薄膜法(蛋白纯度均值97.1%)测定结果相近,统计学分析显示2种方法的测定结果无显著性差异(P>0.05)。结论:琼脂糖凝胶电泳法的精密度和耐用性良好,可用于白蛋白纯度测定。
Objective:To establish the method of determination of albumin purity by agarose gel electrophoresis. Methods:Helana Epalyzer 2 automatic electrophoresis instrument and its supporting kit were used,concentration of test sample was 2%,concentration of staining solution was 0.35%,volume of staining solution was 800 μL,and loading volume of the tets sample was 50 μL. 153 batches of albumin samples were measured by this method and compared with the results of the cellulose acetate film method. Results:The quantitative determination range of the agarose gel electrophoresis method was 1%-5%,and the lowest detection concentration was 0.1%. The RSD of precision examination was less than 0.5%. The results of this method(average protein purity was 96.7%)were similar to those of the cellulose acetate film method(average protein purity was 97.1%). Statistical analysis showed there was no significant difference between the two methods(P>0.05). Conclusion:Agarose gel electrophoresis has good precision and durability and can be used for albumin purity determination.
作者
柯兵兵
汪模正
巫建翔
郭江红
KE Bing-bing;WANG Mo-zheng;WU Jian-xiang;GUO Jiang-hong(Hubei Institute for Drug Control&Hubei Engineering Research Center for Drug Quality Control,Wuhan 430073,China;Tonglu Bio-Pharmaceutical Co.,Ltd.,Hefei 230000,China)
出处
《药物分析杂志》
CAS
CSCD
北大核心
2019年第12期2279-2285,共7页
Chinese Journal of Pharmaceutical Analysis
关键词
琼脂糖凝胶电泳
醋酸纤维素薄膜电泳
人血白蛋白
杂蛋白
纯度
agarose gel electrophoresis
cellulose acetate membrane electrophoresis
human albumin
impurity protein
purity
