摘要
目的:探讨FOXP1和PBRM1在结肠癌组织以及癌旁组织中表达情况,分析其与结肠癌患者临床病理特征及预后的关系。方法:选取75例结肠癌患者癌组织及对应癌旁组织,采用qRT-PCR法检测FOXP1和PBRM1基因表达水平,免疫组织化学法检测FOXP1和PBRM1蛋白表达,使用Kaplan-Meier方法计算FOXP1和PBRM1表达对结肠癌患者存活率的影响,Spearman相关性分析FOXP1和PBRM1表达之间的相关性,Cox回归模型分析结肠癌预后的影响因素。结果:与癌旁组织相比,结肠癌组织中FOXP1基因表达水平上调,PBRM1基因表达水平下调,差异均具有统计学意义(P<0.01)。结肠癌组织中FOXP1和PBRM1阳性表达率分别为65.33%和41.33%,癌旁组织中FOXP1和PBRM1阳性表达率分别为24.00%和77.33%。与癌旁组织相比,结肠癌组织中FOXP1阳性表达明显上升,PBRM1阳性表达明显下降,差异具有统计学意义(P<0.05)。FOXP1和PBRM1在结肠癌组织中的表达均与患者的年龄、性别以及肿瘤大小无关(P﹥0.05),而与TNM分期、分化程度和淋巴结转移相关(P<0.05)。FOXP1阳性表达患者存活率显著低于阴性表达患者(P<0.05),PBRM1阳性表达患者存活率显著高于阴性表达患者(P<0.05),FOXP1阳性表达患者存活率明显低于PBRM1阳性表达患者(P<0.05)。Spearman相关性分析结果表明,FOXP1和PBRM1表达呈负相关(r=-0.547,P<0.05)。Cox回归模型分析结果显示,FOXP1和PBRM1均是影响结肠癌预后的独立因素。结论:FOXP1和PBRM1在结肠癌中表达异常,两者均参与结肠癌发生、发展过程,可能成为结肠癌诊治的新肿瘤标记物。
Objective:To investigate the expression of FOXP1 and PBRM1 in colon cancer tissues and paracancerous tissues,and to analyze their relationships with clinicopathological characteristics and prognosis of colon cancer.Methods:The colon cancer tissues and corresponding paracancerous tissues of 75 patients with colon cancer were selected.The expression levels of FOXP1 and PBRM1 genes were detected by qRT-PCR.The protein expression of FOXP1 and PBRM1 was detected by immunohistochemistry.The influence of FOXP1 and PBRM1 expression on survival rate of colon cancer patients was calculated by Kaplan-Meier.Correlation between FOXP1 and PBRM1 expression was analyzed by Spearman correlation analysis.Cox regression model was used to analyze the prognostic factors of colon cancer.Results:Compared with paracancerous tissues,the expression level of FOXP1 gene in colon cancer tissues was up-regulated,and the expression level of PBRM1 gene was down-regulated,with statistical significances(P<0.01).The positive expression rates of FOXP1 and PBRM1 in colon cancer tissues were 65.33%and 41.33%respectively,and the positive expression rates of FOXP1 and PBRM1 in paracancerous tissues were 24.00%and 77.33%respectively.Compared with paracancerous tissues,the positive expression of FOXP1 in colon cancer tissues increased significantly,and the positive expression of PBRM1 decreased significantly,with statistical significances(P<0.05).The expression of FOXP1 and PBRM1 was not correlated with age,gender and tumor size(P>0.05),but was correlated with TNM stage,differentiation degree and lymph node metastasis(P<0.05).The survival rate of patients with FOXP1 positive expression was significantly lower than that of patients with negative expression(P<0.05),and the survival rate of patients with PBRM1 positive expression was significantly higher than that of patients with negative expression(P<0.05).The survival rate of patients with FOXP1 positive expression was significantly lower than that of PBRM1 positive expression patients(P<0.05).Spearman c
作者
李永良
Li Yongliang(Gastrointestinal Surgery Department,the Affiliated Hospital of Putian University,Fujian Putian 351100,China)
出处
《现代肿瘤医学》
CAS
2020年第5期770-775,共6页
Journal of Modern Oncology
