摘要
目的了解为期一年的监测中广州市水产品创伤弧菌和河弧菌污染水平、菌株携带毒力基因和分子分型情况。方法对广州市水产品中创伤弧菌和河弧菌进行分离鉴定,毒力基因鉴定和肠道细菌基因间重复序列聚合酶链式反应(Eric-PCR)。结果创伤弧菌阳性率为10.4%(31/298),河弧菌为5.0%(15/298);用PCR法检测创伤弧菌毒力溶血素基因vvhA全部阳性,vcgC/E和16S rRNA A/B分型结果显示,共有CB型、EA型和CA型3种基因型别。河弧菌毒力相关基因vfh、toxR全部阳性,hupO携带率为60.0%(9/15)、vfp携带率为80.0%(12/15);Eric-PCR扩增出8~14条100~2 000 bp之间的条带,将15株河弧菌在相似系数为0.8处分为5个群11个类型。结论广州市水产品中创伤弧菌和河弧菌污染情况较严重,大部分菌株携带毒力基因,Eric-PCR结果显示15株河弧菌在亲缘关系上具有相关性,应加强防控。
Objective To investigate the contamination, virulence-associated genes and molecular typing of Vibrio vulnificus and Vibrio fluvibrio in aquatic products of Guangzhou City. Methods Vibrio vulnificus and Vibrio fluvibrio suspected isolates were further identified by VITEK-2 system and polymerase chain reaction(PCR). The virulence-associated genes of the Vibrio isolates were determined, and genotyping analysis was performed by enterobacterial repetitive intergenic consensus-polymerase chain reaction(Eric-PCR). Results The positive rates were 10.4%(31/298) and 5.0%(15/298) for Vibrio vulnificus and Vibrio fluvibrio. Three genotypes of Vibrio vulnificus were detected, including CB, EA and CA. The virulence-associated genes vfh and toxR of Vibrio fluvibrio were all positive, the detection rate of hupO was 60.0%(9/15) and vfp was 80.0%(12/15). Eric-PCR amplified 8-14 bands between 100-2 000 bp, and 15 Vibrio fluvibrio strains were divided into 5 groups and 11 types with a similarity coefficient of 0.8. Conclusion The contamination of Vibrio vulnificus and Vibrio fluvibrio in aquatic products in Guangzhou was serious, and most virulence-associated genes were present. Eric-PCR result showed that 15 Vibrio vulnificus strains were related to each other in genetic relationship, so prevention and control should be strengthened.
作者
张晶
和鹏
周勇
陶霞
白志军
吴新伟
ZHANG Jing;HE Peng;ZHOU Yong;TAO Xia;BAI Zhijun;WU Xinwei(Guangzhou Center for Diseases Control and Prevention,Guangdong Guangzhou 510440,China)
出处
《中国食品卫生杂志》
CSCD
北大核心
2019年第6期570-573,共4页
Chinese Journal of Food Hygiene
基金
广州市医药卫生科技项目(20171A011274)
广东省科技计划项目(2016A020215217)
广州市科技计划项目(201707010130)
广州市医学重点学科建设项目(2017-2019-07)
关键词
创伤弧菌
河弧菌
毒力基因
肠道细菌基因间重复序列聚合酶链式反应
Vibrio vulnificus
Vibrio fluvibrio
virulence-associated genes
enterobacterial repetitive intergenic consensus-polymerase chain reaction
