摘要
高原鼠兔是青藏高原特有的小型哺乳动物,其繁殖活动呈现明显的季节性。成年雄性高原鼠兔在繁殖期睾丸重量显著增加,精子发生正常进行,而在非繁殖期睾丸退化,精子发生阻断在未分化精原细胞阶段。光周期控制实验显示,长光照(16 h∶8 h)诱导非繁殖期高原鼠兔重新启动精原细胞分化和精子发生;而短光照(8 h∶16 h)显著抑制繁殖期高原鼠兔精子发生。酶联免疫分析发现,褪黑素分泌水平在长日照条件下降低而在短日照条件下升高。非繁殖期高原鼠兔连续注射褪黑素拮抗剂能诱导生殖细胞发育和精子发生恢复。促性腺激素释放激素(GnRH)与黄体生成素(LH)在繁殖期鼠兔下丘脑垂体显著升高,促卵泡素(FSH)水平无显著差异。注射GnRH可以促进非繁殖期高原鼠兔精原细胞分化和精子发生,而褪黑素注射后抑制GnRH的分泌进而负调控性腺轴。综上,高原鼠兔季节性精子发生受光周期—褪黑素信号控制,后者主要通过控制GnRH、LH水平影响精原细胞分化。本研究对理解季节性动物精子发生的调控机制有重要借鉴意义。
The plateau pika(Ochotona curzoniae),a small mammal native to the Qinghai-Tibetan Plateau,breed in summer and become reproductively dormant in winter.In this study,we found stimulation(16 h∶8 h)induced spermato-gonial differentiation and the resumption of spermatogenesis,while short-day treatment(8 h∶16 h)resulted in spermato-gonial differentiation arrest.Melatonin content was significantly increased in short-day adapted animals and injection of me-latonin inhibited the spermatogonial differentiation and development of advanced germ cells,while treatment with a melato-nin receptor antagonist stimulated spermatogonial differentiation and spermatogenesis in the reproductively dormant animals.Levels of gonadotropin-releasing hormone(GnRH),luteinizing hormone(LH)were significantly increased in reproduc-tively active males while follicle-stimulating hormone(FSH)did not differ.Functional experiments revealed that GnRH injection stimulated germ cells development and initiation of spermatogenesis in non-breeding season.Furthermore,GnRH content in brain was decreased by melatonin administration.Together,these data demonstrated that the seasonal spermato-genesis of plateau pikas was directed by photoperiod-melatonin signal.These findings revealed a key mechanism underlying seasonal reproduction in plateau pikas and likely other animal species。
作者
王玉军
贾功雪
王婷
王秀蓉
刘忠浩
都玉蓉
马建滨
WANG Yujun;JIA Gongxue;WANG Ting;WANG Xiurong;LIU Zhonghao;DU Yurong;MA Jianbin(Key Laboratory of Medicinal Plant and Animal Resources the Qinghai-Tibetan Plateau in Qinghai Province,College of Life and Geography Science,Qinghai Normal University,Xining 810008,China;Key Laboratory of Evolution and Adaptation of Plateau Biota,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810001,China;Qinghai Province Key Laboratory of Animal Ecological Genomics,Xining 810001,China)
出处
《兽类学报》
CAS
CSCD
北大核心
2020年第1期54-63,共10页
Acta Theriologica Sinica
基金
国家自然科学基金(31760117,31700341)
青海省科技厅基础研究计划项目(2019-ZJ-924)
青海省青藏高原药用动植物资源重点实验2017-ZJ-Y13
青海省动物基因组学重点实验室2017-ZJ-Y23
支持“率先行动”中国博士后科学基金会与中国科学院联合资助优秀博士后项目
关键词
高原鼠兔
季节性繁殖
光周期
褪黑素
Plateau pika
Seasonal reproduction
Photoperiod
Melatonin
