摘要
秋季，将布氏田鼠置于２３±２℃，光周期１４Ｌ∶１０Ｄ的环境中，每日腋下注射褪黑激素，共四周。导致布氏田鼠的非颤抖性产热被激活，褐色脂肪组织和肝的总蛋白及线粒体蛋白含量明显地增加；肝线粒体状态４和状态３呼吸活力显著增加；刺激肝和褐色脂肪组织线粒体细胞色素Ｃ氧化酶活力升高；明显地升高血清Ｔ３浓度和增加Ｔ３／Ｔ４比率。表明在秋季褪黑激素能明显提高布氏田鼠的产热能力。短光照对布氏田鼠产热的调控可能是通过松果体分泌的褪黑激素而实现的。
In autumn Brandt′s voles were randomly divided into three groups kept at 23±2 ℃: 1) Long day control group (LC), 14 L∶10 D 2) Short day control group (SC), 10 L∶14 D, both LC and SC received daily subaxillary injection of 0 1 ml of 1% ethanolic saline for 4 weeks. 3) Melatonin treated group (LM),14 L∶10 D,which received daily subaxillary injection of melatonin (0 1 ml of 1% ethanolic saline containing 12 5 μg melatonin) for 4 weeks. The results showed that resting metabolic rate (RMR) and nonshivering thermogenesis (NST) of Brandt′s voles increased during exposure to short photoperiod, and melatonin significantly activated NST of Brandt′s voles. Short day and melatonin obviously induced an increase in the contents of total protein and mitochondrial protein of liver and brown adipose tussue(BAT),a rise in of state 4 and state 3 respiration of liver and an increase in the cytochrome C oxidase activities in liver and BAT mitochondria, but did not significantly stimulate the α glycerophosphate oxidase activity of BAT.Serum T 3 content and the ratio of T 3 to T 4 were obviously enhanced by melatonin and short day. These results indicate that thermogenic capacities of Brandt′s voles are markedly stimulated by melatonin. Melatonin secreted by the pineal gland may be involved in the short day thermoregulation.
出处
《动物学报》
SCIE
CAS
CSCD
1998年第1期20-26,共7页
ACTA ZOOLOGICA SINICA
基金
国家自然科学基金
关键词
布氏田鼠
光照周期
褪黑激素
产热
Brandt′s voles ( Microtus brandti ), Photoperiod, Melatonin, Mitochondria, Nonshivering thermogenesis
