摘要
目的:探讨银翘散对流感病毒感染所致重症肺炎小鼠的修复机制。方法:选择小鼠60只,随机分为模型组(对照组)及治疗组(观察组),各30只,建立流感病毒感染所致重症肺炎小鼠模型,观察组给予银翘散治疗,观察两组小鼠的肺组织病理学改变情况;比较两组小鼠的肺组织湿干比(W/D)、总肺水含量(TLW)情况。Western blot法检测肺组织NF-κB表达情况;ELISA法检测不同时期炎症因子(IL-6、IL-1、TNF-α)水平情况。结果:HE结果显示,对照组的肺组织病理损伤明显比观察组更严重,观察组小鼠肺组织病理评分明显低于对照组(P<0.05)。干预治疗3、5、7d后,观察组NF-κB表达水平明显低于对照组,IL-6、IL-1、TNF-α的浓度明显低于对照组(P<0.05)。结论:银翘散对流感病毒感染所致重症肺炎小鼠肺组织具有修复炎性病变的作用,其可能通过抑制炎症因子分泌来实现。
Objective:To investigate the repair mechanism of Yinqiaosan on mice with severe pneumonia caused by influenza virus infection.Methods:Sixty mice were selected and randomly divided into a model group(control group)and a treatment group(observation group),30 mice each.A mouse model of severe pneumonia caused by influenza virus infection was established.The observation group was treated with Yinqiaosan.The pathological changes of lung tissue in the mice of the group were compared;the wet-to-dry ratio(W/D)and total lung water content(TLW)of the lung tissue of the two groups of mice were compared.Western blot was used to detect the expression of NF-κB in lung tissue;ELISA was used to detect the levels of inflammatory factors(IL-6,IL-1,TNF-α)in different periods.Results:The HE results showed that the lung tissue pathological damage in the control group was significantly more severe than that in the observation group,and the lung tissue pathological score of the observation group was significantly lower than that in the control group(P<0.05).After 3,5,and 7 days of intervention,the expression levels of NF-κB in the observation group were significantly lower than those in the control group,and the concentrations of IL-6,IL-1,and TNF-αwere significantly lower than those in the control group(P<0.05).Conclusion:Yinqiaosan can repair inflammatory lesions in mice with severe pneumonia caused by influenza virus infection,which may be achieved by inhibiting the secretion of inflammatory factors.
作者
王佳
冯星火
国丽娜
王妍
WANG Jia;FENG Xinghuo;GUO Lina(Liaoning Provincial People’s Hospital,Shenyang 110000)
出处
《陕西中医》
2020年第2期156-159,共4页
Shaanxi Journal of Traditional Chinese Medicine
基金
“十三五”国家科技重大专项课题(2017ZX10305501005)
国家自然科学基金资助项目(81403271)
