摘要
本文通过网络药理学探讨荔枝核抗炎作用机制。通过TCMSP及CTD数据库获取荔枝核与炎症相关的潜在基因靶点,采用String数据库构建蛋白相互作用网络图,运用Cytoscape 3.8.0软件构建荔枝核活性成分-靶点-通路网络图,利用WebGestalt对荔枝核潜在的关键靶点分别进行GO和KEGG的富集分析。结果表明,荔枝核中具有抗炎作用的关键活性成分有3个,即quercetin、betasitosterol和Stigmasterol,涉及包括TP53、EGF、RB1、CDKN1A、CASP9、VEGFA和CXCL8等31个核心靶点;通过调节Pathways in cancer signaling pathway、Human cytomegalovirus infection signaling pathway和HepatitisB signaling pathway等信号通路来发挥抗炎作用,从而得出结论构建的"活性成分-靶点-通路"网络图,展示了荔枝核通过多成分、多靶点、多通路的形式来发挥抗炎作用,可为荔枝核抗炎的进一步研究提供参考。
In this paper,the anti-inflammatory mechanism of Litchi Semen seeds was discussed by the network pharmacology.The potential gene targets related to inflammation in Litchi Semen seeds were obtained by TCMSP and CTD database.The String database was used to construct the protein interaction network map.Cytoscape 3.8.0 was used to construct the active component-targets-pathway network map of litchi nucleus.GO and KEGG enrichment analysis of potential key targets in Litchi Semen seeds were conducted by Web Gestalt.The results showed that there were 3 key anti-inflammatory active components in Litchi Semen seeds,including quercetin,beta-sitosterol and Stigmasterol;Involving 31 core targets including TP53,EGF,RB1,CDKN1 A,CASP9,VEGFA and CXCL8,and exerting their anti-inflammatory effects by regulating pathways in cancer signaling pathway,Human cytomegalovirus infection signaling pathway,Hepatitis B signaling pathway and other signal pathways.It is concluded that the"active ingredient-target-pathway"network diagram shows the anti-inflammatory effect of Litchi Semen seeds in the form of multi-components,multi-targets and multi-pathways,which would provide a reference for further research on anti-inflammation of Litchi Semen seeds.
作者
李玉凤
丁世磊
赵立春
LI Yu-feng;DING Shi-lei;ZHAO Li-chun(Guangxi University of Chinese Medicine,Nanning 530200,China;Guangxi Zhuang yao Medicine Center of Engineering and Technology,Nanning 530200,China.)
出处
《特产研究》
2021年第3期50-58,64,共10页
Special Wild Economic Animal and Plant Research
基金
广西中医药大学首届岐黄工程高层次人才团队项目(2018002)
广西中医药大学“一流学科”资助课题(2018XK033)
广西高等学校高水平创新团队及卓越学者项目(2019-52)。
关键词
荔枝核
抗炎
网络药理学
作用机制
Litchi Semen seeds
anti-inflammatory
network pharmacology
mechanism
