摘要
通过批次实验探究生物炭对苯酚厌氧降解产甲烷过程的促进机制,并考察了300,500,700℃下制备的生物炭对苯酚甲烷化过程延滞期、最大产甲烷速率和微生物群落结构的影响.结果表明:生物炭的电子交换能力与苯酚甲烷化过程具有显著关系(R2=0.997).与对照组相比,投加15g/L的生物炭可将苯酚甲烷化的延滞期从15.0d缩短至1.1~3.2d,最大产甲烷速率由4.0mL/d提高到10.4~13.9mL/d.其中在热解温度500℃下制备的生物炭由于含有丰富的电化学活性类醌结构,对苯酚甲烷化过程促进效果最优.此外,生物炭投加促进了典型产电细菌Geobacter及产甲烷菌Methanosaeta的富集.进一步说明生物炭投加通过促进种间电子传递加速了苯酚甲烷化过程.
The potential mechanisms for anaerobic phenol degradation and methanogenesis promotion by biochar addition were evaluated by batch experiments,and the effects of biochar derived at 300,500 and 700℃on lag time,maximum methane production rate and microbial community structure were elucidated in this study.The results indicated a strong linear relationship between the electron exchange capacity of biochar and Rmax(R2=0.997).Furthermore,methanogenic lag time was notably shortened from 15.0days to 1.1~3.2days,and the maximum CH4 production rate was increased from 4.0mL/d to 10.4~13.9mL/d by biochar addition(15g/L).Moreover,the methanogenesis of phenol was optimized by addition of biochar prepared at 500℃because of the most abundance of electrochemically active quinones.In addition,microbial community analysis showed that the electroactive Geobacter and Methanosaeta microbial consortia were enriched by biochar addition.It was further demonstrated that biochar addition fueled the methane production rate of phenol by promoting interspecies electron transfer.
作者
高新
王高骏
李倩
陈荣
GAO Xin;WANG Gao-jun;LI Qian;CHEN Rong(School of Environmental&Municipal Engineering,Xi’an University of Architecture and Technology,Xi’an 710055,China)
出处
《中国环境科学》
EI
CAS
CSCD
北大核心
2020年第2期631-639,共9页
China Environmental Science
基金
国家自然科学基金资助项目(51608430,51978560)
国家重点研发项目(2017YFE0127300)
陕西省创新团队项目(2019TD-025)。
关键词
生物炭
苯酚降解
甲烷化
直接种间电子传递
biochar
phenol degradation
methanogenesis
interspecies electron transfer
