摘要
目的探讨益肾通癃胶囊对前列腺增生(benign prostatic hyperplasia,BPH)模型大鼠雌雄激素比及缺氧诱导因子1α(chypoxia-inducible fac tor-1α,HIF-1α)的影响。方法对SD雄性大鼠皮下注射丙酸睾酮5 mg/(kg·d),连续4周。造模成功后将大鼠随机分为4组:正常组、模型组、中药对照组和实验组,每组7只。实验组大鼠灌服0.365 g/kg益肾通癃胶囊混悬液;中药对照组灌服0.183 g/kg癃闭舒胶囊混悬液;正常组及模型组给予同等容量的生理盐水灌胃,连续灌胃8周后,光镜下观察前列腺组织病理学变化;称取前列腺湿重并计算前列腺指数;检测大鼠血清E2、T、DHT水平及E2/T;测定前列腺组织中HIF-1α蛋白表达。结果与模型组比较,实验组大鼠前列腺腺体轻微增生,少数呈乳头状增生,腺腔基本恢复正常。与模型组比较,实验组大鼠前列腺体积、前列腺湿重、前列腺指数及前列腺组织中的HIF-1α蛋白表达水平均降低(P<0.01);大鼠血清中的DHT、E2、T水平降低,E2/T比值升高(P<0.01)。结论益肾通癃胶囊可能通过降低BPH模型大鼠血清中的DHT、E2、T水平,升高E2/T比值,抑制HIF-1α的表达起治疗BPH的作用。
Objective To investigate the effects of Yishen Tongzhi Capsules on the estrogen and androgen ratio in rat model of benign prostatic hyperplasia and the effects on hypoxia-inducible factor-1α(HIF-1α). Methods SD male rats were injected subcutaneously with testosterone propionate 5 mg/(kg·d) for 4 weeks. After successful modeling, the rats were randomly divided into4 groups: a normal group, a model group, a Chinese medicine control group and an experimental group, with 7 rats in each group.Rats in the experimental group were administrated with 0.365 g/kg Yishen Tonglong Capsules Suspension;the Chinese medicine control group was administrated with 0.183 g/kg Longbishu Capsules Suspension;the normal group and the model group were given the same volume of saline. After 8 weeks of continuous intragastric administration, the histopathological changes of prostate were observed under light microscope;The prostate wet weight was measured and the prostate index was calculated;The rat serum E2,T, DHT levels, and E2/T were detected;HIF-1α protein expression in prostate tissue was measured. Results Compared with the model group, the prostate glands of the experimental group had a slight hyperplasia, and a few showed papillary hyperplasia.The glandular cavity returned to normal. Compared with the model group, rats in the experimental group had reduced prostate volume, wet prostate weight, prostate index, and HIF-1α protein expression levels in prostate tissue(P<0.01);DHT, E2, and T levels in rat serum were reduced. E2/T ratio increased(P<0.01). Conclusion Yishen Tonglong Capsules may treat BPH by reducing the levels of DHT, E2, T in the serum of BPH model rats, increasing the E2/T ratio and inhibiting the expression of HIF-1α.
作者
涂雅玲
陈其华
TU Yaling;CHEN Qihua(Hunan University of Chinese Medicine,Changsha,Hunan 410208,China;The First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha,Hunan 410007,China)
出处
《湖南中医药大学学报》
CAS
2020年第1期14-17,共4页
Journal of Hunan University of Chinese Medicine
基金
湖南省科技厅科技创新平台与人才计划-男性疾病中医临床医学研究中心（2018SK4012）
2018年湖南省研究生科研创新项目（CX2018B494）
关键词
前列腺增生
益肾通癃胶囊
雌雄激素比
缺氧诱导因子
prostatic hyperplasia
Yishen Tonglong Capsules
estrogen and androgen ratio
hypoxia-inducible factor
