摘要
为探究莼菜多糖组成及其抗氧化活性,以4大产区商品莼菜嫩叶为实验材料,采用苯酚-硫酸法测定莼菜总糖含量,结果显示雷波莼菜的总糖含量最高,为(15.470±0.348)mg/g;利用高效液相色谱法(performance liquid chromatography,HPLC)检测莼菜多糖组成及含量,鉴定出10种单糖成分,主要为半乳糖(36.05%~40.33%)、岩藻糖(14.32%~16.68%)和甘露糖(11.60%~16.35%)。采用DPPH、FRAP、ABTS三种体外抗氧化检测方法测定莼菜多糖的抗氧化活性,发现莼菜水溶性多糖抗氧化能力强于碱溶性多糖,综合抗氧化能力强弱顺序为:利川莼菜>石柱莼菜>雷波莼菜>西湖莼菜。相关性分析表明,多糖浓度与抗氧化活性密切相关,甘露糖等多种单糖具有抗氧化活性。这为我国莼菜研究和资源开发提供更多的基础数据。
Young leaves of commodity Brasenia schreberi from four production areas were used as experimental material in this experiment to explore the polysaccharide composition of Brasenia schreberi and its antioxidant activity.The total polysaccharide content of Brasenia schreberi was determined by phenol-sulfuric acid method.The result showed that the total polysaccharide content of Leibo Brasenia schreberi was the highest:(15.470±0.348)mg/g.The components and contents of polysaccharide in Brasenia schreberi were detected by HPLC,where ten monosaccharide components were identified,and the major ones were galactose(36.05%-40.33%),fucose(14.32%-16.68%)and mannose(11.60%-16.35%).The antioxidant activity of polysaccharide from Brasenia schreberi was determined by DPPH,FRAP and ABTS in vitro.It was found that water-soluble polysaccharide has stronger antioxidant capacity than alkal-soluble polysaccharide in Brasenia schreberi,and the comprehensive antioxidant capacity was in the order of Lichuan>Shizhu>Leiboi>Xihu.Correlation analysis showed that polysaccharide concentration was closely related to antioxidant activity and mannose and some other monosaccharides had antioxidant activity.This study provides more basic data for Brasenia schreberi research and resource development in China.
作者
李京凌
滕左
韩芳
袁婧
于杰
LI Jingling;TENG Zuo;HAN Fang;YUAN Jing;YU Jie(College of Horticulture and Landscape Architecture,Southwest University,Chongqing 400715,China;Tongliang No.2 Middle School,Chongqing 402560,China;Key Laboratory of Horticulture Science for Southern Mountainous Regions,Ministry of Education,Chongqing 400715,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2020年第1期262-268,共7页
Food and Fermentation Industries
基金
国家自然科学基金(31772260)
重庆市社会民生项目(cstc2017shms-kjfp80009)
西南大学科技创新基金(SZ201802)
关键词
莼菜
水溶性多糖
碱溶性多糖
高效液相色谱
抗氧化活性
Brasenia schreberi
water-soluble polysaccharides
alkal-soluble polysaccharides
HPLC
antioxidant activity
