摘要
利用RT-PCR方法从巴西蕉(Musa acuminata L.AAA group,cv.Brazilian)中克隆MaARF2基因,并对其进行序列及表达分析。基因克隆结果获得该基因编码片段,命名为MaARF2,全长2655 bp,编码884个氨基酸,分子量为97917.38 Da,理论等电点pI为6.64,序列富含丝氨酸、脯氨酸,亲水性氨基酸多于疏水性氨基酸并均匀分布在整个肽链中;通过Motif Search工具发现了ARF基因所特有的B3、Auxin_resp、AUX_IAA family结构域;多序列比对和进化树分析表明,MaARF2基因编码的蛋白与其他植物中ARF基因编码的蛋白具有较高的一致性。qRT-PCR结果表明,MaARF2在香蕉根、茎、叶、花和果实中均表达,其中叶片中表达水平最高,果实表达量最低;MaARF2在低温、盐和干旱胁迫后表达量均上调,表明其可能参与调控香蕉低温、盐和干旱胁迫响应的过程。本研究首次在香蕉中克隆了MaARF2基因,为进一步研究该基因的生物学功能奠定了基础。
An auxin response factor gene from banana designated as MaARF2 was amplified by RT-PCR,and its sequence and expression were analyzed.Sequence analysis indicated that the length of the MaARF2 ORF was 2655 bp,encoding 884 amino acids,the protein molecular weight was 97917.38 Da,and the theoretical isoelectric point pI was 6.26.The amino acid sequence encoded by this gene was rich in serine and proline,and the hydrophilic amino acid was more than the hydrophobic amino acid and evenly distributed in the whole peptide chain.Through the Motif Search tools three domains including B3,Auxin_resp,and AUX_IAA family conformed to the structural features of ARF were found.Multiple sequence alignment and phylogenetic tree analysis showed that the protein encoded by MaARF2 was highly consistent with the protein encoded by ARF in other plants.qRT-PCR results showed that MaARF2 was expressed in banana roots,stems,leaves,flowers and fruits,among which the expression level in leaves was the highest and the expression level in fruits was the lowest.The expression of MaARF2 was up-regulated after low temperature,salt and drought stress,indicating that MaARF2 may be involved in the regulation of responses to these stresses in bananas.MaARF2 was firstly cloned in this study,which would lay a foundation for further research on the biological function of this gene.
作者
黄东梅
许奕
吴斌
马伏宁
陈弟
李敬阳
林妃
宋顺
HUANG Dongmei;XU Yi;WU Bin;MA Funing;CHEN Di;LI Jingyang;LIN Fei;SONG Shun(Haikou Experimental Station,Chinese Academy of Tropical Agricultural Sciences/Hainan Key Laboratory of Banana Genetic Improvement,Haikou,Hainan 571101,China)
出处
《热带作物学报》
CSCD
北大核心
2020年第1期89-96,共8页
Chinese Journal of Tropical Crops
基金
海南省自然科学基金项目(No.317226)
国家科技支撑计划子课题“柑桔、香蕉等(亚)热带果树优质高效生产关键技术研究与示范”(No.2014BAD16B01)
关键词
香蕉
生长素响应因子
克隆
序列分析
banana
auxin response factor
clone
sequence analysis
