摘要
目的探讨转移相关蛋白2(metastasis-associated protein 2,MTA2)在喉鳞状细胞癌组织中的表达,分析其与临床病理特征和患者预后的关系。方法采用免疫组化SP法检测120例喉石蜡包埋组织中MTA2蛋白的表达,采用Western blot法检测喉新鲜组织中MTA2蛋白的表达量,利用Kaplan-Meier法绘制总生存期曲线,Log-rank方法分析两组生存曲线差异,Cox比例风险回归模型筛选喉鳞状细胞癌患者的预后影响因素。结果MTA2在正常喉组织、高级别鳞状上皮内病变和喉鳞状细胞癌组织中的阳性率分别为6.67%、43.33%和73.33%,差异有统计学意义(χ^2=36.11,P<0.001),MTA2表达与喉鳞状细胞癌组织学分级、临床分期及淋巴结转移显著相关(P均<0.05);在正常喉组织、高级别鳞状上皮内病变及喉鳞状细胞癌组织中MTA2的相对表达量分别是0.98±0.07、1.30±0.07和1.46±0.11,差异有统计学意义(F=121.194,P<0.001);Kaplan-Meier生存分析提示MTA2表达与喉鳞状细胞癌患者的生存期显著相关(P<0.05);多因素回归分析表明MTA2蛋白表达、临床分期及淋巴结转移是喉鳞状细胞癌患者有效的独立预后指标(P均<0.05)。结论MTA2过表达在喉鳞状细胞癌发生、进展过程中发挥重要作用,可作为喉鳞状细胞癌独立的预后因素,MTA2有望成为指导喉鳞状细胞癌治疗和判断患者预后的新分子学标志物。
Purpose The aim of this study was to detect MTA2 expression in laryngeal squamous cell carcinoma(LSCC)and to analyze its association with clinicopathological factors and prognosis of LSCC patients.Methods The level of MTA2 expression in 120 paraffin-embedded specimen tissues was determined by immunohistochemistry,and in fresh specimens by Western blot.The overall survival curve was calculated using the Kaplan-MeieRmethod,and the differences between the two groups was analysed by Log-rank method.Cox proportional hazards regression model was used to examine the independent predictive factors in the patients with LSCC.Results The positive rate of MTA2 expression in normal laryngeal tissues,high-grade squamous intraepithelial lesion(HSIL)and LSCC were 6.67%,43.33%,73.33%,respectively,and there were significant difference(χ^2=36.11,P<0.001).The expression of the MTA2 was significantly correlated to histological grade,clinical stage and lymph node metastasis(all P<0.05).The relative expression of MTA2 in normal laryngeal tissues,Hsil and LSCC were 0.98±0.07,1.30±0.07,1.46±0.11,respectively,the difference was statistically significant(F=121.194,P<0.001).Kaplan-MeieRsurvival analysis showed a significant correlation between MTA2 expression and patient survival rate(P<0.05).Multivariate regression analysis revealed that the expression of MTA2,clinical stage and lymph node metastasis were independent prognostic factors foRclinical outcome of LSCC(all P<0.05).Conclusion The oveRexpression of MTA2 may play an important role in the tumorigenesis and progression of LSCC and was an independent prognostic factor.MTA2 can be used as a new biomarkeRfoRthe therapy and prognosis of LSCC patients.
作者
刘玉东
甄娟
韩晓丽
郭明丽
LIU Yu-dong;ZHEN Juan;HAN Xiao-li;GUO Ming-li(Department of Otolaryngology,Hebei General Hospital,Shijiazhuang 050057,China;Department of Pathology,Shijiazhuang Fourth Hospital,Shijiazhuang 050000,China)
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2020年第1期33-37,共5页
Chinese Journal of Clinical and Experimental Pathology
基金
河北省医学科学研究重点课题计划(20190346)
关键词
喉肿瘤
喉鳞状细胞癌
MTA2
临床病理特征
预后
laryngeal neoplasm
laryngeal squamous cell carcinoma
MTA2
clinicopathological factors
prognosis
