摘要
目的:观察电针对胰岛素抵抗肥胖(OIR)大鼠机体炎性反应状态和胰岛素敏感性的影响,探讨电针干预胰岛素抵抗的机制。方法:Wistar雄性大鼠随机选取13只作为正常组,给予普通饲料喂养,其余给予高脂饲料喂养。8周后符合肥胖标准的大鼠随机分为模型组、电针组、假电针组,每组13只。电针组取"足三里""中脘""关元""丰隆"穴,连接电针仪治疗,每周3次,每次15 min,共治疗8周;假电针组于电针组腧穴旁开约5 mm处浅刺并夹持电极但不通电,其余同电针组。造模8周后测定各组大鼠的葡萄糖输注速率(GIR);于干预第6周检测腹腔糖耐量(IPGTT)和腹腔胰岛素耐量(IPITT);治疗结束后取心尖血用酶联免疫吸附法检测血清中胰岛素(INS)和C反应蛋白(CRP)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)的水平;Western blot法和实时荧光定量PCR法检测脂肪组织中IL-6、TNF-α、IL-10、IL-1β、单核细胞趋化蛋白-1(MCP-1)蛋白和mRNA表达水平;免疫组织化学法检测脂肪组织中CD68的表达。结果:与正常组比较,模型组GIR水平显著下降,IPGTT和IPITT中血糖值及血清INS、CRP、IL-6、TNF-α水平均显著升高,脂肪组织中IL-6、TNF-α、IL-1β、MCP-1蛋白和mRNA表达显著增高,IL-10蛋白和mRNA表达显著下降,CD68的表达明显增高(P<0.01,P<0.05)。与模型组比较,电针组GIR水平显著升高,IPGTT和IPITT中血糖值及血清INS、CRP、IL-6、TNF-α水平显著下降,脂肪组织中IL-6、TNF-α、IL-1β、MCP-1蛋白和mRNA表达显著降低,IL-10蛋白和mRNA表达显著升高,CD68的表达明显减少(P<0.01,P<0.05);干预后假电针组与模型组比较,各指标差异无统计学意义(P>0.05)。结论:电针可以降低OIR大鼠机体炎性反应状态,提高机体胰岛素敏感性。
Objective To observe the effect of electroacupuncture(EA)on inflammatory reaction and insulin sensitivity in insulin-resistant obese(OIR)rats.MethodsThirteen male Wistar rats were randomly selected as the control group and fed with common diet.The other 39 rats were fed with high-fat diet for 8 weeks to establish OIR model and then randomized into model,EA and sham EA groups.EA(2 Hz,1 mA)was applied to unilateral"Zusanli"(ST36),"Fenglong"(ST40),"Zhongwan"(CV12)and"Guanyuan"(CV4)for 15 min,once every other day for 8 weeks,and sham EA was applied to unilateral 4 control spots about 5 mm lateral to the aforementioned 4 acupoints after shallowly inserting acupuncture needles,but without electric current output.After 8 weeks’intervention,the body weight was recorded and the glucose infusion rate(GIR)measured using hyperinsulinemic-euglycemic clamp technique.At the 6 th week of intervention,glucose contents of intraperitoneal glucose tolerance test(IPGTT)and intraperitoneal insulin tolerance test(IPITT)were measured.The levels of serum insulin(INS)and inflammatory factors as C-reactive protein(CRP),IL-6 and TNF-αwere measured by using ELISA at the end of the treatment.The expression levels of IL-6,TNF-α,monocyte chemoattractant protein-1(MCP-1),IL-10 and IL-1βproteins and mRNAs in the abdominal adipose tissues were detected by Western blot and quantitative real time-PCR,separately.The CD68 expression(displaying infiltration of macrophages)of adipose tissue was detected using immunohistochemistry.ResultsAfter modeling,the contents of glucose of IPGTT at 30,60 and 120 min and those of IPITT at 15,30,60 and 120 min,serum INS,CRP,IL-6 and TNF-α,as well as the expression levels of IL-6,TNF-α,IL-1βand MCP-1 proteins and mRNAs and CD68 protein were significantly increased(P<0.01,P<0.05),while the levels of GIR and IL-10 protein and mRNA were obviously decreased in the model group in comparison with those of the control group(P<0.01),suggesting an increase of inflammation and a decline of INS sensitivity.Following the
作者
黄琪
宋燕娟
于兆民
任加凤
梁凤霞
陈瑞
徐芬
HUANG Qi;SONG Yan⁃juan;YU Zhao⁃min;REN Jia⁃feng;LIANG Feng⁃xia;CHEN Rui;XU Fen(College of Acupunc⁃ture⁃moxibustion and Orthopedics⁃traumatology,Hubei University of Chinese Medicine,Wuhan 430061,China;Department of Rehabilitation,Central Hospital of Wuhan,Tongji Medical College of Huazhong University of Science and Technology,Wuhan 430014;Department of Integrated Traditional Chinese and Western Medicine,Union Hospital,Tongji Medical College of Hua⁃zhong University of Science and Technology,Wuhan 430022)
出处
《针刺研究》
CAS
CSCD
北大核心
2019年第12期898-905,共8页
Acupuncture Research
基金
国家自然科学基金项目(No.81574065、81774420、81603699)
关键词
电针
肥胖
胰岛素抵抗
脂肪组织
炎性反应
Acupuncture
Obesity
Insulin resistance
Adipose tissue
Inflammatory reaction
