摘要
目的探讨糖尿病脑病患者外周血血清和单核细胞中miRNA-368水平的表达变化及临床意义。方法 qRT-PCR检测糖尿病脑病中患者外周血血清的miRNA-368表达水平,并将miRNA-368转染到糖尿病脑患者外周血单核细胞中,然后通过qRT-PCR检测转染效率,采取CCK-8和克隆形成实验检测miRNA-368对糖尿病脑患者外周血单核细胞增殖的影响,流式细胞技术检测miRNA-368对糖尿病脑患者外周血单核细胞周期和凋亡率的影响。结果 27例糖尿病脑病患者外周血中miRNA-368的相对表达量为1.402,95%CI=0.363~0.595;miRNA-368相对表达水平与患者的一般资料如年龄、性别并不存在明显关系(P>0.05);糖尿病脑病患者的miRNA-368相对表达水平明显上调(P<0.05);qRT-PCR显示糖尿病脑病外周血单核细胞miRNA-368的表达水平明显上调;si-miRNA-368干预后糖尿病脑病患者外周血单核细胞增殖受到抑制,凋亡率增高。结论 miRNA-368在糖尿病脑病患者外周血血清和单核细胞中的表达水平明显上调,且miRNA-368能抑制单核细胞的凋亡,促进了糖尿病脑病的发生发展。
Objective To study the expression level change and clinic significance of miRNA-368 in peripheral blood of patients with diabetic encephalopathy. Methods qRT-PCR was used to detect the expression level of miRNA-368 in peripheral blood of patients with diabetic encephalopathy in Sanya. MiRNA-368 was transfected into peripheral blood mononuclear cells collected from patients. The transfection efficiency was detected by qRT-PCR. The effect of miRNA-368 on the proliferation of peripheral blood mononuclear cells in diabetic encephalopathy patients was detected by CCK-8 and clonogenicity assay. The effect of miRNA-368 on the peripheral blood mononuclear cell cycle and apoptotic rate in diabetic encephalopathy patients was analyzed by flow cytometry. Results The relative expression level of miRNA-368 in 27 patients with diabetic encephalopathy was 1.402, and the 95% CI was 0.363~0.595. The relative expression level of miRNA-368 was not related to the general data of patients such as age and gender(P> 0.05), while the relative expression level of miRNA-368 in diabetic encephalopathy patients was significantly increased(P<0.05). The relative expression level of miRNA-368 was significantly correlated with that of diabetic encephalopathy. QRT-PCR showed that miRNA-368 expression level was significantly up-regulated in the serum and peripheral blood mononuclear cells of patients with diabetic encephalopathy. After the intervention of si-miRNA-368, the proliferation of peripheral blood mononuclear cells in diabetic encephalopathy patients was inhibited and the apoptosis rate increased. Conclusion The expression level of miRNA-368 in the serum and peripheral blood mononuclear cells of diabetic encephalopathy patients was significantly up-regulated, and miRNA-368 could inhibit the apoptosis of blood mononuclear cells in patients with diabetic encephalopathy, which might promote the development of diabetic encephalopathy.
作者
孟开顺
宋科秀
王小蕊
Meng Kaishun;Song Kexiu;Wang Xiaorui(Sanya City People's Hospital,Sanya 572000)
出处
《卒中与神经疾病》
2019年第6期706-709,共4页
Stroke and Nervous Diseases
