摘要
为了明确桦褐孔菌发酵产物(Inonotus obliquus fermentation products,IOFP)饲喂时间对鸡新城疫(Newcastle disease,ND)疫苗免疫效果的影响,优化和完善饲喂方案,降低生产成本,对饲喂不同时间IOFP机体的体液和细胞免疫反应部分指标进行了测定。试验分为6组,在日粮中全程添加(Ⅰ组)、免疫前添加7 d(Ⅱ组)、免疫前后分别添加4 d和3 d(Ⅲ组)、每添加4 d间隔3 d(Ⅳ组)8 g/kg IOFP,且均免疫新城疫病毒(Newcastle disease virus,NDV)弱毒疫苗;Ⅴ组免疫接种疫苗,饲喂商品化日粮;Ⅵ组为以PBS代替疫苗免疫,同时饲喂商品化饲料的空白对照组。所有动物于14日龄进行NDV弱毒疫苗免疫,21 d后加强免疫。分别于首免后0、7、14、21、28、35、42 d分离各组外周血血清,进行NDV血凝抑制(hemagglutination inhibition,HI)抗体滴度和中和抗体滴度的测定;分离不同时间点外周血淋巴细胞(peripheral blood mononuclear cell,PBMC)进行增殖指数的测定、Th1型和Th2型细胞因子mRNA表达水平的检测。结果显示,在日粮中添加IOFP能够有效提升疫苗的免疫效果,相比于其他各组,Ⅰ组和Ⅱ组的添加方式能够更为显著的提升机体的NDV HI抗体滴度和中和抗体滴度、PBMCs增殖水平和Th1型/Th2型细胞因子mRNA的表达量。考虑到成本,最终选择免疫前饲喂7 d为IOFP作为口服免疫增强剂的最佳应用方式。
In order to illuminate the effects of different Inonotus obliquus fermentation products(IOFP)feeding times on immune efficacy of Newcastle disease vaccine in chickens and subsequently optimize the feeding scheme,which could reduce the running costs,partial humoral and cellular immune indexes were detected.In this study,six groups were assigned:chickens in four groups were orally administered 0.8%IOFP in diets for the whole time(groupⅠ),seven consecutive days before each immunization(groupⅡ),four and three days before and after each immunization respectively(groupⅢ)and four days interval three days(groupⅣ);chickens in groupⅤwere fed with commercial diets and groupⅥwere control group that inoculated with PBS instead of vaccine and also fed with commercial diets.All animals were vaccinated with Newcastle disease virus(NDV)La Sota live vaccine via intranasal and eye-dropped route at 14 days of age,and boosted two weeks later.At 0,7,14,21,28,35 and 42 days post-inoculation(dpi)firstly,the temporal changes in serum Newcastle disease hemagglutination inhibition(HI)and neutralizing antibody titers were determined.Meanwhile,proliferations of peripheral blood mononuclear cells(PBMCs)isolated from each group in response to concanavalin A stimulation and the expression levels of Th1-type and Th2-type cytokines were detected.Results showed that oral administration of IOFP could significantly enhance the effect of vaccination.Compared with other groups,ND HI and neutralizing antibody titers,proliferation of PBMCs,as well as the expression levels of Th1 and Th2 cytokines were superior in groupⅠandⅡ.Together with the cost factor,the best feeding way of IOFP was supplemented in diet for seven consecutive days before each immunization.
作者
白俊平
吴家强
于森
杨少华
林冬梅
邱文彬
蒋万春
张琳
BAI Jun-ping;WU Jia-qiang;YU Sen;YANG Shao-hua;LIN Dong-mei;QIU Wen-bin;JIANG Wan-chun;ZHANG Lin(Institute of Animal Science and Veterinary Medicine,Shandong Academy of Agricultural Science,Shandong Key Lab of Disease Control and Breeding,Jinan,Shandong,250100,China;College of Life Sciences,Shandong Normal University,Jinan,Shandong,250014,China;College of Life Sciences and Food Engineering,Hebei University of Engineering,Handan,Hebei,056021,China)
出处
《动物医学进展》
北大核心
2019年第12期24-29,共6页
Progress In Veterinary Medicine
基金
“十二五”农村领域国家科技计划项目(2015BAD12B03)
山东省农业科学院农业科技创新工程项目(CXGC2016B14,CXGC2018 E10)
泰山学者特聘专家工程经费(ts201511069)
