摘要
目的研究有氧运动联合姜黄素对ob/ob糖尿病小鼠胰岛素抵抗及肝病理性变化的影响,同时检测其对肝脂肪酸β氧化信号通路的影响,探讨其作用的具体机制。方法将32只ob/ob糖尿病小鼠分为糖尿病对照组(ON组),有氧运动干预组(OA组),姜黄素干预组(OC组),有氧运动+姜黄素干预组(OAC组),另外8只C57/BL6J作为正常对照组(NC组)。实验过程中对OA组和OAC组小鼠实施每周6天的有氧运动游泳训练;对OC组和OAC组小鼠给予姜黄素治疗(50μg/(g·d));所有小鼠干预均持续8周后,检测小鼠葡萄糖耐受和胰岛素抵抗情况;肝的病理性变化以及肝中脂肪酸β氧化信号通路的变化情况。结果经过实验干预后,OA组、OC组和OAC组胰岛素敏感性显著增强,其中OAC组几乎达到正常对照NC组水平;OA组、OC组和OAC组血清中反应肝功能的指标TC和TG均低于糖尿病对照ON组,肝功能明显改善;糖尿病小鼠的肝纤维化水平得到明显缓解,OA组、OC组和OAC组显著低于糖尿病对照ON组,OAC组与NC组无显著性差异;肝中脂肪酸合成相关基因的表达没有显著变化,但OA组、OC组和OAC组小鼠肝中脂肪酸β氧化相关基因的表达显著高于ON组,并且OAC组显著高于OA组和OC组;Western blot结果显示OA组、OC组和OAC组小鼠肝中脂肪酸β氧化途径中限速酶肉碱脂酰转移酶Ⅰ(carnitine palmityl transferaseⅠ,CPT-I)的表达显著高于ON组。结论有氧运动联合姜黄素可以促进糖尿病小鼠肝中脂肪酸β氧化关键限速酶CPT-I的表达,从而促进脂肪酸β氧化的发生,增强脂质分解,改善肝的病理变化,并且增强胰岛素敏感性,为2型糖尿病的治疗提供了新的思路。
Objective To study the effects of aerobic exercise combined with curcumin on insulin resistance and liver pathological changes in ob/ob diabetic mice,to determine the effects of curcumin combined with aerobic exercise on the hepatic fatty acidβ-oxidation signal pathway,and to explore the specific mechanisms behind these effects.Methods Thirty-two ob/ob diabetic mice were divided into a diabetic control group(ON group),aerobic exercise intervention group(OA group),curcumin intervention group(OC group),and aerobic exercise+curcumin intervention group(OAC group),while another eight C57/BL6 J mice served as a normal control group(NC group).During the experiment,aerobic exercise training was performed on OA group and OAC group mice.Curcumin treatment(50μg/(g·d))was applied to OC group and OAC group mice.After 8 weeks of intervention,glucose tolerance and insulin resistance of the mice were determined.The pathological changes and fatty acidβ-oxidation signaling pathways in the liver were also measured.Results After the experimental intervention,the insulin sensitivity of the OA group,OC group,and OAC group was significantly increased,and the OAC group almost reached the level of the NC group;the serum TC and TG of the OA group,OC group,and OAC group were lower than those of the ON group,and the liver function was significantly improved;the liver fibrosis level of diabetic mice was significantly improved,and these levels of the OA group,OC group,and OAC group were significantly lower than those of the NC group.There was no significant difference between the OAC group and the NC group in the diabetic control group.There was also no significant change in the expression of fatty acid synthesis-related genes in the liver,but the expression of fatty acidβ-oxidation-related genes in the OA group,OC group,and OAC group was significantly higher than that in the ON group,and the expression of fatty acidβ-oxidation-related genes in the OAC group was significantly higher than that in the OA group and OC group.Western blottin
作者
刘晓晨
张社峰
王改凤
LIU Xiaochen;ZHANG Shefeng;WANG Gaifeng(Department of Physical Education,Henan Finance University,Zhengzhou 450046,China;Department of Endocrine,Henan Province Chinese Medicine Research Institute Affiliated Hospital,Zhengzhou 450004;First Department of Encephalopath,Henan Province Hospital of TCM,Zhengzhou 450002)
出处
《中国比较医学杂志》
CAS
北大核心
2020年第1期29-35,共7页
Chinese Journal of Comparative Medicine
基金
2018年河南省体育局课题:体医结合在河南省城乡居民公共服务中的现状与对策研究(2018026)
河南省科技攻关项目:糖尿病神经病变“玄关络虚”病机及临床研究(172102310019)
河南省省属科研机构预研专项:祛风补肾方调控AMPK-Mtor信号改善高糖诱导的足细胞损伤机制研究(201704570)
