摘要
采用SSR分子标记技术鉴定两系杂交水稻“荃两优2118”的种子纯度。从48对SSR引物中筛选到2组引物(RM7120、RM8277)。随机选取“荃两优2118”种子6组,每组样品随机取192棵种子苗种进行纯度检测,结果显示同一样品SSR标记与田间鉴定结果接近,最大、最小差异分别为2.05%和0.23%。因此,SSR标记技术适用于“荃两优2118”的种子纯度鉴定。
The seed purity of two-line hybird rice“Quan liangyou 2118”was identified using simple sequence repeat(SSR)molecular markers.Two pairs of primers(RM7120 and RM8277)were screened out from 48 pairs of SSR primers.A total of 2 pairs of primers were used to test the purity of 6 groups of the“Quan liangyou 2118”,with 192 seed seedings in each group.The SSR identification results were basically agreed with the field identification results,the difference of which ranged from 0.23%-2.05%.The research results indicated that SSR markers could be utilized to identify the purity of“Quan liangyou 2118”.
作者
王立广
张翔
黄贯刘
汪仁举
康圣好
熊延军
张奎
WANG Li-guang;ZHANG Xiang;HUANG Guan-liu(Anhui Hua’an Seed Co.,Ltd.,Hefei,Anhui 230031)
出处
《安徽农业科学》
CAS
2020年第1期42-43,共2页
Journal of Anhui Agricultural Sciences
关键词
SSR分子标记
两系杂交水稻
种子纯度
田间鉴定
Simple sequence repeat(SSR)molecular markers
Two-line hybrid rice
Seed purity
Field identification
