摘要
目的:探讨地中海贫血患者血清miR-609水平及其临床预后价值。方法:选择自2017年4月至2018年4月在本院接受治疗的地中海贫血患者127例,同时选取100例体检健康者为对照组,采用RT-PCR实验分析miR-609变化,分析miR-609与地中海贫血临床指标的关系,采用多因素Logistic回归分析评估地中海贫血预后的危险因素。结果:地中海贫血患者中miR-609相对表达量为3.17±0.24,显著高于对照组(P<0.05)。miR-609高表达患者ALT、Plt、MCH水平明显高于miR-609低表达患者(P<0.05)。miR-609高表达患者Hb、sICAM-1水平明显低于miR-609低表达患者(P<0.05)。miR-609水平与患者的性别、年龄、AST无相关性(P>0.05)。miR-609高表达组患者的轻度贫血发生率明显低于miR-609低表达的患者(P<0.05)。miR-609水平与中度贫血发生率无相关性(P>0.05)。miR-609高表达组的严重贫血患者例数明显多于miR-609低表达的患者(P<0.05)。miR-609高表达组的地中海贫血患者头晕、乏力及发热发生率均明显高于miR-609低表达的患者(P<0.05)。miR-609水平与地中海贫血患者恶心发生率无相关性。ROC曲线发现,miR-609的AUC值为0.862,灵敏度为83.6%,特异度为84.1%。这提示miR-609对地中海贫血有较高的诊断价值。多因素Logistic回归分析发现,MCH、miR-609是地中海贫血患者预后不良危险因素。结论:地中海贫血患者血清miR-609水平上升,是患者的预后不良危险因素,可作为评估患者疗效的参考指标。
Objective:To investigate the level of serum microRNA-609 and its clinical prognostic value in patients with thalassemia.Methods:One hundred and twenty-seven patients with thalassemia treated in our hospital from April2017 to April 2018 were selected,100 healthy persons were selected as control group.The changes of miR-609 were analyzed by RT-PCR,the relationship between miR-609 and clinical indicators of thalassemia was analyzed,and the prognostic risk factors of thalassemia were evaluated by multivariate logistic regression analysis.Results:The relative expression level of miR-609 in thalassemia patients was 3.17±0.24,which was significantly higher than that in control group(P<0.05).The levels of ALT,Plt and MCH in patients with high expression of miR-609 were significantly higher than those in patients with low expression of miR-609(P<0.05).The levels of Hb and sICAM-1.in patients with high expression of miR-609 were significantly lower than those in patients with low expression of miR-609(P<0.05).There was no correlation between the level of miR-609 and the patient's sex,age and AST(P>0.05).The incidence rate of mild anemia in high expression group was significantly lower than that in low expression group(P<0.05).There was no correlation between the level of miR-609 and the incidence rate of moderate anemia(P>0.05).The number of patients with severe anemia in the miR-609 high expression group was higher than that in miR-609 low expression group(P<0.05).The incidence rate of dizziness,fatigue and fever in patients with miR-609 high expression group was significantly higher than those in patients with miR-609 low expression(P<0.05).There was no correlation between the level of miR-609 and the incidence rate of nausea in patients with thalassemia.ROC curve showed that the AUC value of microRNA-609 was 0.862,the sensitivity was 83.6%,and the specificity was 84.1%,which suggested that miR-609 had a high diagnostic value for thalassemia.Multivariate logistic regression analysis showed that MCH and mir-609 were risk
作者
王明泉
程凌利
高晶晶
谢仁伟
林丽屏
杨建忠
许荣誉
WANG Ming-Quan;CHENG Ling-Li;GAO Jing-Jing;XIE Ren-Wei;LIN Li-Ping;YANG Jian-Zhong;XU Rong-Yu(Department of Blood Transfusion,Quanzhou First Hospital Affiliated to Fujian Medical University,Quanzhou 362000,Fujian Province,China;Department of Hematology,Quanzhou First Hospital Affiliated to Fujian Medical University,Quanzhou 362000,Fujian Province,China;Department of Oncology,Quanzhou First Hospital Affiliated to Fujian Medical University,Quanzhou 362000,Fujian Province,China)
出处
《中国实验血液学杂志》
CAS
CSCD
北大核心
2019年第6期1933-1937,共5页
Journal of Experimental Hematology
