摘要
对唐古拉哈日钦冰芯表层0.04~3.04m及深层127.44~130.36m中可培养细菌进行了对比研究。发现表层和深层可培养细菌数没有表现出显著差异性,表层冰芯中可培养细菌数为0~9.8×10^3CFU·mL^-1,略高于深层冰芯的0~8.4×10^3CFU·mL^-1。表层冰芯中微生物总数为10^3~10^6cells·mL^-1,深层冰芯中微生物总数为10^2~10^3cells·mL^-1。冰芯中可培养细菌属于Firmicutes(厚壁菌门)、Proteobacteria(变形菌门)、Actinobacteria(放线菌门)、Bacteroidetes(拟杆菌门)和Deinococcus-Thermus(异常球菌门)5大类。但表层和深层可培养细菌属于不同的优势门,表层为Proteobacteria(38%),深层为Firmicutes(42%)。表层和深层优势属均为Bacillus(芽孢杆菌属),占比分别为18%和29%。已有研究表明哈日钦冰芯98.8m达到了公元1000年的历史记录,因此对比千年尺度上可培养细菌数量及多样性的差异,能够为进一步发掘新基因,丰富微生物多样性,为了解可培养细菌的进化历史奠定基础。
A comparative study was carried out on the culturable bacteria groups in the surface layer(0.04-3.04 m depth)and the deep layer(127.44-130.36 m depth)of the Tanggula Hariqin ice core. It was found that there was no significant difference in the number of culturable bacteria between the surface and deep layers.The number of culturable bacteria in the surface was 0-9.8×10^3 CFU·mL^-1,slightly higher than that of deep ice core 0-8.4×10^3 CFU·mL^-1. The total microorganisms cell count in the surface was 103-106 cells·mL^-1,while that in the deep layer was 10^2-10^3 cells·mL^-1. The culturable bacteria in ice core belonged to the five phyla,namely Proteobacteria,Firmicutes,Actinobacteria,Bacteroidetes and Deinococcus-Thermus. However,culturable bacteria at the surface and deep layer belonged to different dominant phylum,the surface is Proteobacteria(38%)and the deep layer is Firmicutes(42%). Nevertheless,Bacillus was the most dominant genus in both layers,with the abundance of 18% and 29% in surface and deep layers,respectively. The previous study shows the 98.8 m depth ice core of Hariqin reached 1000 A. D. historical record. Comparing the differences in the number and diversity of culturable bacteria on the millennium scale,it can further explore new genes,enrich microbial diversity and lay a foundation for understanding the evolution history of culturable bacteria.
作者
王凤
王宁练
徐柏青
沈亮
顾政权
刘晓波
WANG Feng;WANG Ninglian;XU Baiqing;SHEN Liang;GU Zhengquan;LIU Xiaobo(Key Laboratory of Alpine Ecology,Institute of Tibetan Plateau Research,Chinese Academy of Sciences,Beijing 100101,China;College of Urban and Environmental Science,Northwest University,Xi'an 710069,China;Key Laboratory of Tibetan Environment Changes and Land Surface Process,Institute of Tibetan Plateau Research,Chinese Academy of Sciences,Beijing 100101,China;CAS Center for Excellence in Tibetan Plateau Earth Sciences,Beijing 100101,China;University of Chinese Academy of Sciences,Beijing 100049,China;Kathmandu Center for Research and Education,Chinese Academy of Sciences,Kathmandu 44618,Nepal)
出处
《冰川冻土》
CSCD
北大核心
2019年第4期968-976,共9页
Journal of Glaciology and Geocryology
基金
国家自然科学基金项目（41425004）资助
关键词
青藏高原
哈日钦冰芯
可培养细菌
表层
深层
Tibetan Plateau
Hariqin ice core
culturable bacteria
surface layer
deep layer
