摘要
采用液体培养,考察碳源、氮源、Cu^2+及其他培养条件对血红栓菌产漆酶的影响。采用2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐法测定血红栓菌菌株所产漆酶的酶活力。结果表明,以葡萄糖15 g·L^-1和蔗糖15 g·L^-1为碳源,蛋白胨2 g·L^-1和硝酸铵2 g·L^-1为氮源,CuSO42.0 mmol·L^-1,初始pH 5.0,300 mL三角瓶装液量为100 mL,接种量8%,温度28℃,在此条件下,发酵液漆酶酶活为165.19 U·mL^-1,比优化前的53.80 U·mL^-1提高了3倍。
The effect of carbon resource,nitrogen source,Cu^2+ and other culture conditions on laccase by liquid fermentation cultivation of the Trametes sanguinea were studied.The laccase production of T.sanguinea was detected by 2,2’-azinobis-(3-ethylbenzthiazoline-6-sulphonate)method.The results showed that,the optimum conditions were glucose 15 g·L^-1,sucrose 15 g·L^-1,peptone 2 g·L^-1,ammonium nitrate 2 g·L^-1,CuSO42.0 mmol·L^-1,and the initial pH was 5.0,liquid medium volume was100 mL per 300 mL,the inoculation quantity was 8%and the temperature was 28℃.Under these conditions,the maximal laccase activity was 165.19 U·mL^-1,which was 3 times higher than that before optimization(53.80 U·mL^-1).
作者
郭金英
赵翠敏
张云龙
王宁宁
郑素月
GUO Jin-ying;ZHAO Cui-min;ZHANG Yun-long;WANG Ning-ning;ZHENG Su-yue(College of Landscape and Ecological Engineering,Hebei University of Engineering,Handan 056021,China)
出处
《中国食用菌》
北大核心
2019年第11期52-56,共5页
Edible Fungi of China
基金
河北省现代农业产业技术体系食用菌创新团队建设专项资金（HBCT2018050202）
关键词
血红栓菌
漆酶
培养条件
Trametes sanguinea
laccase
culture conditions
