摘要
利用基础产酶培养基从保藏的9株白腐真菌中筛选得到一株高产漆酶菌株毛栓孔菌XYG422,并通过单因素试验对该菌株发酵培养基及培养条件进行优化筛选,获得较高产漆酶能力,同时研究了该菌株对玉米秸秆的生物降解。研究结果表明:在液体发酵条件下,XYG422产漆酶最适宜碳氮源成分为玉米粉和酒石酸铵,菌株XYG422发酵条件优化后产漆酶酶活显著提升,该菌株最佳发酵培养条件为:玉米粉40g/L、酒石酸铵3g/L、温度30℃、pH 8.0、接种量5个直径1cm的菌饼、转速180r/min,诱导剂吐温-80和2,5-二甲基苯胺在低浓度时对菌株产漆酶有明显的促进作用,菌株产漆酶活性最高可达到41.6U/m L。该菌株表现出了对玉米秸秆较好的生物降解效率,培养60d后,玉米秸秆中木质素、纤维素和半纤维素的降解率依次为83.54%、50.65%和19.53%。
Trametes hirsuta XYG422 with high laccase activity was selected by liquid fermentation from nine white-rot fungal stocks. Single-factor experiment was designed to optimize the laccase producing ability according to different cultural media and conditions. Then, corn straws were used to assess the degradation by this strain. The XYG422 laccase production were corn flour and tartaric acid ammonium. The optimiz op ed timized nitrogen sources for nutritional ingredients and fermentation conditions were corn flour 40g/L, tartaric acid ammonium 2g/L, incubation temperature of 30℃, culture medium pH value of 8.0, inoculum quantity of five fungal agar plugs （diameter 1cm） and rotary rate of 180r/min. Tween-80 and 2,5-dimethyl aniline at low concentration promoted laccase production. Under the optimized conditions, the laccase activity produced in XYG422 was significantly increased up to 41.6U/mL. Trametes hirsuta XYG422 exhibited the best corn straw biodegradation effect. After 60d inoculation, the lignin degradation rate reached 83.54%, and the cellulose and hemi-cellulose degradation rate was 50.65% and 19.53% respectively.
作者
甄静
李冠杰
李伟
杜志敏
王继雯
尹文兵
陈国参
ZHEN Jing LI Guan-Jie LI Wei DU Zhi-Min WANG Ji-Wen YIN Wen-Bing CHEN Guo-Can(Key Laboratory of Microbial Engineering of Henan, Zhengzhou, Henan 450008, China Biology Institute of Henan Academy of Sciences, Zhengzhou, Henan 450008, China Stare Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, 8eijing 100101, China)
出处
《菌物学报》
CAS
CSCD
北大核心
2017年第6期718-729,共12页
Mycosystema
基金
应用微生物技术联合专项基金(2015)
北京市自然科学基金(5152018)
河南省科学院预研项目(2015)~~
关键词
白腐真菌
漆酶
发酵优化
玉米秸秆
生物降解
white-rot fungi, laccase, fermentation optimization, corn straw, biodegradation
