摘要
初步探究玫瑰茄水提物的抗贫血作用机制。建立IDA大鼠模型,随机分成4组,对IDA大鼠灌胃低、中、高剂量的玫瑰茄水提物。30d后,解剖大鼠,取十二指肠和肝脏。利用Q-PCR和Westernblot技术分别检测试验大鼠十二指肠中二价金属转运体(DMT-1)、膜铁转运蛋白(FPN-1)、膜铁转运辅助蛋白(Hp)及其mRNA的表达量,肝脏铁调素(Hepcidin)及其mRNA的表达水平。结果表明,与低铁对照组比较,玫瑰茄水提物低、中剂量组对DMT-1、FPN-1、Hp表达量的下调幅度不超过9%,mRNA的表达水平则超过50%,低、中剂量均对Hp mRNA无显著性差异;高剂量组对DMT-1、FPN-1、Hp及其mRNA表达量的下调程度不一。低剂量组Hepcidin-25的表达量是低铁对照组的1.61倍;高剂量组的表达量则下调了65.79%;各剂量组均抑制了相关mRNA的表达。因此,玫瑰茄水提物通过提供有效成分促进机体铁的吸收,从而影响IDA大鼠的DMT-1、FPN-1、Hp、Hepcidin-25及其mRNA的表达,维持机体铁稳态。
Preliminary study was conducted on the mechanism underlying the anti-anemia function of an aqueous extract of Hibiscus sabdariffa L.(HS). The IDA rat model was established and the resulting rats were randomly divided into 4 groups. The IDA rats were administered with low, medium and high doses of HS. After 30 days, the rats were dissected and their duodenum and liver were taken. Then, Q-PCR and Western blot techniques were used to detect the expression levels of divalent metal transporter 1(DMT-1), ferroportin(FPN), hephaestin(Hp) and their mRNA in the duodenum as well as the expression of hepcidin and its mRNA in the liver of the rats. The results showed that the decreases in the expression levels of DMT-1, FPN-1 and Hp for the low and medium HS dose groups were not more than 9%, while the mRNA expression level were over 50%, compared with those of low-iron control group. There were no significant difference in Hp mRNA between the low dose and medium dose groups. The decreases in the expression of DMT-1, FPN-1, Hp and their mRNA induced by the high-dose HS varied. The expression level of Hepcidin-25 in the low-dose group was 1.61 times as high as that for the low-iron control group, whilst the expression level in the high-dose group decreased by 65.79%. HS at different doses all showed an inhibitory effect on the mRNA expression. Therefore, HS promotes the absorption of iron in the body by providing active constituents, thereby affecting the expression of DMT-1, FPN-1, Hp, Hepcidin-25 and its mRNA in IDA rats, to maintain the iron homeostasis inside the body.
作者
曾荣
方舒婷
赵文俊
林淇淇
何锡媛
王嫣如
ZENG Rong;FANG Shu-ting;ZHAO Wen-jun;LIN Qi-qi;HE Xi-yuan;WANG Yan-ru(College of Food Science and Engineering,Foshan University,Foshan 528200,China;Guangdong Provincial Key Laboratory of Emergency Test for Dangerous Chemicals,Guangdong Institute of Analysis,Guangzhou 510070,China)
出处
《现代食品科技》
EI
CAS
北大核心
2019年第11期16-24,共9页
Modern Food Science and Technology
基金
佛山市植物功能性食品研究科技创新平台
佛山科学技术学院校级大学生创新创业计划项目(XJ2019231)
