摘要
目的探讨水飞蓟素(SM)对人肝癌细胞系MHCC97侵袭和转移能力的影响及可能涉及的分子机制。方法MTT法检测细胞增殖;Transwell、划痕实验和细胞-基质黏附实验分别检测细胞侵袭、迁移及黏附能力;RT-PCR和Western blot检测MMP-9、VEGF、E-cadherin、integrinβ1和Akt mRNA及蛋白的表达。结果和对照组相比,水飞蓟素抑制细胞增殖(P<0.05),显著降低细胞侵袭、迁移和黏附能力(P<0.05)。水飞蓟素处理组MMP-9、VEGF、integrinβ1和Akt的mRNA及蛋白表达量降低(P<0.05),而E-cadherin的mRNA及蛋白表达量增高(P<0.05)。结论水飞蓟素可抑制MHCC97细胞增殖,并且降低细胞侵袭、迁移和黏附能力。
Objective To study the effects of silymarin on invasion and migration of MHCC97 cell lines,and to explore the possible mechanism.Methods Cell suppression rate was detected by MTT assay.Migration,invasion and adhesion of MHCC97 cells after silymarin treatment were observed by cell migration assay,wound healing assay and cell-matrix adhesion assay.The mRNA and protein levels of MMP9,VEGF,E-cadherin,integrinβ1 and Akt were detected by reverse transcription polymerase chain reaction(RT-PCR)and Western blot separately.Results The silymarin suppressed growth of MHCC97 cell,migration,invasion and adhesion.The mRNA and protein level of MMP-9,VEGF,Integrinβ1 and p-Akt were also reduced,but the expressions of mRNA and protein of E-cadherin increased.Conclusions Silymarin can inhibit the growth of MHCC97 cells,and reduce the invasion,migration,and adhesion abilities of MHCC97 cell lines.
作者
张健
马海
杨柳
ZHANG Jian;MA Hai;YANG Liu(the First Department of General Surgery,Nanchong 637000,China;Department of Respiration,Nanchong Central Hospital,Nanchong 637000,China)
出处
《基础医学与临床》
CSCD
2019年第12期1741-1745,共5页
Basic and Clinical Medicine
