摘要
为探究苦参叶绿体基因组特征及该属物种的系统进化发育关系,本研究利用高通量测序技术对苦参叶绿体基因组进行测序和功能注释。结果显示:苦参叶绿体基因组全长154165 bp,呈典型的四段式结构。苦参叶绿体基因组共包含123个基因,包括77个蛋白编码基因、38个tRNA基因及8个rRNA基因。经序列分析鉴定出104个SSR位点,大部分重复由A和T组成。此外该叶绿体基因组密码子偏好性较弱,编码区偏向使用A和T碱基。对两个不同地区苦参叶绿体基因组进行比较分析发现了4个差异基因。基于最大似然法(ML)对苦参及其他16种豆科植物进行系统发育分析,发现苦参与其同属植物苦豆子的亲缘关系最近。本研究为苦参的遗传变异、育种以及系统发育分析等提供了重要的理论依据,具有一定的参考价值。
In order to explore the chloroplast genome characteristics of Sophora flavescens and the phylogenetic relationship of the genus,this study used high-throughput sequencing technology to sequence and functionally annotate the chloroplast genome of Sophora flavescens.The results showed that the full length 154165 bp of Sophora flavescens chloroplast genome showed a typical four-stage structure.The chloroplast genome contains123 genes,including 77 protein-coding genes,38 tRNA genes and 8 rRNA genes.Sequence analysis revealed104 SSR loci,most of which consisted of A and T.In addition,the chloroplast genome codon preference is weak,and the coding region is biased towards the use of A and T bases.A comparative analysis of two different regions of Sophora flavescens chloroplast genome revealed four differential genes.Based on the maximum likelihood method(ML)for phylogenetic analysis of Sophora flavescens and 16 other leguminous,it was found that the relationship between Sophora flavescens and the genus Sophora alopecuroides is the closest.This study provides an important theoretical basis for the genetic variation,breeding and phylogenetic analysis of Sophora flavescens,and has certain reference value.
作者
乔永刚
贺嘉欣
王勇飞
曹亚萍
贾孟君
张鑫瑞
梁建萍
宋芸
QIAO Yong-gang;HE Jia-xin;WANG Yong-fei;CAO Ya-ping;JIAMeng-jun;ZHANG Xin-rui;LIANG Jian-ping;SONG Yun(College of Life Sciences,Shanxi Agricultural University,Taigu 030801,China)
出处
《药学学报》
CAS
CSCD
北大核心
2019年第11期2106-2112,共7页
Acta Pharmaceutica Sinica
基金
山西振东企业国际合作项目资助(2018ZDGH0101)
山西省高等学校教学改革项目资助(J2015028,J2017031)
山西省基础研究计划资助(201801D121204)
