摘要
应用RAPD技术,探讨甘草属(G ly cy rrh iza L.)13种1变种30个植物类群的遗传差异和几个争议种的分类地位。从60个随机引物中筛选出14个多态性好的引物进行RAPD实验,DNA片段的二态数据用U PGM A聚类法构建系统发育树。共扩增出250条带,多态性带204条,约占总数的81.7%。聚类结果显示RAPD分子标记构建的系统发育树与经典分类系统一致。甘草属植物具有丰富的遗传多样性,同种内不同居群间的遗传分化较大。黄甘草、胀果甘草、乌拉尔甘草三者亲缘关系较近,平卧甘草与粗毛甘草存在很大的遗传差异,作为独立种较合理。RAPD标记可为甘草属植物的系统分类研究提供分子生物学依据。
RAPD was employed to probe genetic differences and some controversial taxonomies of 30 plant groups belonging to 13 species and 1 variety of Glycyrrhiza L. 14 primers with good polymorphism screened from 60 random primers were used to conduct RAPD analysis and dimorphic data of DNA fragments were used to construct the dendrogram by UPGMA. Totally 250 bands were amplified and 204 of them were polymorphic accounting for 81.7%. Clustering results revealed that the dendrogram constructed with RAPD molecular makers was identical with the classic taxonomic system. Glycyrrhiza L. plant are rich in genetic diversity and different populations in the same species vary much in genetic differentiation. G. eurycarpa P. C. Li,G. inflata Bat. and G. uralensis Fisch are close in genetic relation,and G. prostrata X. Y. Li and G. aspera Pall. genetically differ greatly so that it is reasonable for them to be impendent species. The RAPD markers can provide the basis for taxonomic researches of Glycyrrhiza plants.
出处
《西北植物学报》
CAS
CSCD
北大核心
2006年第3期527-531,共5页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家科技攻关项目-新疆专项(2001EP050012)
石河子大学自然科学基金项目(200310)
关键词
甘草属
RAPD
遗传多样性
分类
Glycyrrhiza L.
RAPD
genetic diversity
classification
