摘要
目的调查安徽安庆地区慢性乙型病毒性肝炎患者所感染的乙型肝炎病毒(HBV)的基因型构成,并探讨不同HBV基因型患者的血清HBV DNA、PreS1、HBeAg检测结果及临床价值,为乙型病毒性肝炎患者的个体化医疗提供依据。方法选取慢性乙肝患者共226例,采用巢式PCR技术对其进行基因分型检测,采用实时荧光定量聚合酶链反应法(RT PCR)检测患者血清HBV DNA,用酶联免疫吸附分析法(ELISA)检测血清PreSl抗原与HBeAg。结果在调查的乙型病毒性肝炎患者中,以B基因型(占58.0%)与C基因型(占41.1%)HBV感染为主。B基因型患者血液中HBV DNA阳性率(57.2%)与C型患者(63.4%)没有统计学差异;B基因型患者血液中PreS1抗原阳性率(48.1%)与C型患者(48.4%)也没有明显差异;C基因型HBV感染的患者血液HBeAg阳性率(46.2%)高于B基因型患者(32.1%)。结论安徽安庆地区在慢性乙肝的卫生防治策略上,应重点关注B、C型基因型HBV感染的防治。而C基因型HBV感染患者具有高HBeAg阳性率,应采取更积极、有效干预措施。
Objective To investigate the distribution of hepatitis B virus(HBV)genotypes in patients with chronic virus hepatitis B in Anqing city of Anhui Province,and study the expression and clinical significances of serum HBV DNA,preS1,HBeAg in various HBV genotypes,s provide the base for individualized treatment of the virus hepatitis B.Methods The HBV genotypes of 226 patients with chronic virus hepatitis B were determined by nested polymerase chain reaction(PCR).The level of serum HBV DNA in patients was detected by Real time quantitative fluorescent polymerase chain reaction(RT qPCR).The levels of serum preS1 and HBeAg were detected by enzyme linked immunosorbent assay(ELISA).Results Among the investigated hepatitis B patients,HBV infection was mainly caused by genotype B(58.0%)and genotype C(41.1%).The positive rate of HBV DNA in the blood of patients with genotype B(57.2%)was not statistically different from that of patients with genotype C(63.4%);the positive rate of PreS1 antigen in the blood of patients with genotype B(48.1%)was not significantly different from that of patients with genotype C(48.4%);the positive rate of HBeAg in the blood of patients with genotype C(46.2%)was higher than that of patients with genotype B(32.1%).Conclusions More attention should be paid in the genotype B,C of HBV infection in the prevention and treatment of chronic virus hepatitis B in Anqing city of Anhui Province.More active and effective intervention measures should be taken to control the genotype C of HBV infection,which has a higher positive rate of HBeAg,higher infection,more serious progression of disease.
作者
吴湃
吴坤河
刘海燕
张斗星
胡安群
WU Pai;WU Kunhe;LIU Haiyan;ZHANG Douxing;HU Anqun(Department of Laboratory Medicine,The affiliated Anqing Hospital,Anhui Medical University,Anqing,Anhui,China,233001;Department of Pathology,Guangdong Women and Children's Hospital,Guangzhou,Guangdong,China,511400)
出处
《分子诊断与治疗杂志》
2019年第6期495-498,507,共5页
Journal of Molecular Diagnostics and Therapy
基金
安徽医科大学科研基金项目(2011xkj092)
