摘要
目的研究了解西安市乙型肝炎病毒(HBV)的基因型分布及其与临床相关性指标丙氨酸氨基转移酶(ALT)和天门冬氨酸转氨酶(AST)关系和临床意义。方法采用巢式PCR对389例西安市HBV感染患者的血清标本进行HBV DNA扩增,后用聚合酶链反应-限制性片段长度多态性法(PCR-RLFP)鉴定HBV基因型。并用酶联免疫吸附试验(ELISA)检测乙肝五项,速率法检测ALT和AST。结果西安市389例HBV感染患者基因型分布为HBV B基因型58例占14.9%(58/389),HBV C基因型326例占83.8%(326/389),HBV B/C混合型5例占1.2%(5/389)。其中HBV B、C基因型与患者在性别和乙型肝炎e抗原(HBeAg)阳性率、HBV DNA载量水平及ALT和AST比较差异均无统计学意义(P>0.05);B、C基因型患者在HBV携带者和慢性乙肝中分布比较差异无统计学意义(P>0.05),但在肝硬化和肝癌中比较差异有统计学意义(P<0.05)。HBV C基因型较B基因型更易发展成为肝硬化甚至肝癌。结论西安市HBV感染者存在HBV B、HBV C、HBV B/C混合型3种基因型,以HBV C为主要基因型,B型次之,B/C混合型最少;肝硬化的发生与C型存在着一定关系,提示HBV C基因型更容易发展成为肝硬化。
Objective To study the relationship and clinical significance of hepatitis B genotype distribution in Xi′an City and clinical relative indicators.Methods The nested PCR was used to amplify HBV DNA in 389 serum samples from HBV infected patients in Xi′an City,then polymerase chain reaction restriction fragment length polymorphism（PCR-RLFP）was used to identify HBV genotypes.Results Among 389 cases of HBV Infection in Xi′an City,58 cases of HBV B genotype accounted for 14.9%（58/389）,326 cases of HBV C genotype accounted for 83.8%（326/389）and 5cases of HBV B/C mixed type accounted for 1.2%（5/389）.The gender,HBeAg positive rate,HBV DNA load level,ALT and AST had no statistical difference between HBV genotype B and C with the patients（P〉0.05）;There was no statistically significant in the distribution of B and C genotypes between the patients with chronic hepatitis B and HBV carriers（P〉0.05）,but it had statistical difference between liver cirrhosis and hepatocellular carcinoma（P〈0.05）.HBV genotype C was easier to develop liver cirrhosis even liver cancer than genotype B.Conclusion The three kinds of HBV genotype B,C and B/C exist in the HBV infected persons in Xi′an City,with the genotype C as the main genotype,genotype B takes the second and mixed genotype B/C is minimal.The liver cirrhosis occurrence has a certain relationship with the genotype C,which suggesting that the genotype C is more likely to develop liver cirrhosis.
出处
《国际检验医学杂志》
CAS
2017年第4期475-477,共3页
International Journal of Laboratory Medicine
关键词
乙肝病毒
分布
基因型
hepatitis B virus
distribution
genotype
