摘要
目的:探讨HBV不同基因型感染患者转归的关系.方法:北京佑安医院住院及门诊1321例肝硬化患者、慢性乙型肝炎患者、慢性乙型肝炎病毒(HBV)携带者和乙型肝炎病毒自限性感染者四组人群进行HBV血清免疫学指标检测、HBVDNA检测、HBV基因型的分析,用HBV特异性基因型引物进行聚合酶链反应(PCR),丙烯酰胺凝胶电泳分析HBVDNA基因型,应用酶联免疫吸附实验方法检测HBV血清免疫学指标.结果:1321例血清标本中804例(60.1%)HBVDNA阳性,HBV基因型分别为A型0.25%、B型20.77%、C型69.53%、B+C混和型9.44%.自限性感染者组14/245例(5.71%)HBVDNA阳性,其中9例C基因型、4例B基因型、1例BC混和基因型;慢性HBV携带者组128/300例(42.67%)HBVDNA阳性,其中88例C基因型、36例B基因型、4例BC混和基因;慢性肝炎患者组608/668例(91.11%)HBVDNA阳性,其中2例A基因型、121例B基因型、417例C基因型、68例B/C混和基因型;肝硬化患者组54/108例(50%)HBVDNA阳性,B型6例、C型45例、B+C混和型3例.肝硬化患者组C基因型为83.33%,分别高于自限性感染者组、慢性HBV携带者组和慢性肝炎患者组(64.29%、68.75%、68.58%,均为P<0.05).年龄,性别与感染HBV基因型无关,HBV感染后病情发展与HBV基因型有关.结论:北京地区乙型肝炎病毒基因型以C型为主,B型次之,其它基因型较少见;肝硬化患者组C基因型分别高于隐性感染者组、慢性乙型肝炎表面抗原携带者组和慢性肝炎患者组;提示乙型肝炎病毒基因型与肝炎病情发展有一定关系.
AIM： To investigate the relationship between the genotypes of hepatitis B virus （HBV） and the clinical outcomes of HBV infection in Beijing area.
METHODS： A total of 1 321 patients, including those with self limited HBV infection （n = 245）, chronic hepatitis B （n = 668）, liver cirrhosis （n = 108） and asymptomatic carders （n = 300）, were concluded in this study. Serum samples were collected for the detection of HBV markers by enzyme linked immunosorbent assay （ELISA）. A simple and precise genotyping system based on polymerase chain reaction （PCR） using typespecific primers was developed for the determination of the genotypes of HBV.
RESULTS： Of the 1 321 serum samples, 804 （60.1%）were HBV DNA positive. HBV with the genotype of B, C, and B＋C covered a larger percent. The percentage of HBV with genotype A, B, C, and B＋C were 0.25% （2/804）, 20.77% （167/804）, 69.53% （559/804） and 9.45% （78/804）, while none of HBV with genotype D, E, and F was found. Of all the patients with self limited HBV infection, 14 were HBV DNA positive, and the rates of genotype B, C, and B＋C were 28.6% （4/14）,64.3% （9/14） and 7.1% （1/14）, respectively. Of the samples from asymptomatic carriers, 128 were HBV DNA positive, and the rates of genotype B, C, and B＋C were 28.1% （36/128）, 68.8% （88/128）, and 3.1% （4/128）, respectively. Of the patients with in chronic hepatitis B, 608 cases were positive for HBV DNA, and the percentage of genotype B, C, B＋C, and A were 19.9% （121/608）, 68.6% （417/608）, 10.4% （68/608）, and 0.3% （2/608）, respectively. Of the patients with liver cirrhosis, 54 were HBV DNA positive, and the percentage of genotype B, C, and B＋C were 11.1% （6/54）, 88.3% （45/54）, and 5.6% （3/54）, respectively. The rate of genotype C in liver cirrhosis patients was markedly higher than that in the patients with chronic hepatitis B, self limited HBV infection, and asymptomatic carders （all P 〈0.05）. In addition, genoty
出处
《世界华人消化杂志》
CAS
北大核心
2005年第24期2823-2827,共5页
World Chinese Journal of Digestology
基金
北京市科技重大项目
No.H020920020590~~
