摘要
目的检测系统性红斑狼疮(SLE)患者血浆白细胞介素(IL)-31、IL-6水平,探讨IL-31水平和自身抗体的关系。方法选取SLE患者42例和健康体检者25例,采用ELISA检测血浆IL-31水平,电化学发光法测定血浆IL-6水平,间接免疫荧光法检测抗核抗体(ANA)水平,免疫印迹法检测自身抗体[抗双链DNA抗体、抗Sm抗体、抗干燥综合征A抗体、抗干燥综合征B抗体、抗核糖体P蛋白抗体、抗干燥综合征(Ro-52)抗体、抗组蛋白抗体、抗核小体抗体、抗核糖核蛋白(RNP)抗体、抗拓扑异构酶抗体、抗着丝点抗体、抗着丝点B抗体]。结果SLE组IL-31和IL-6水平明显高于健康对照组(P<0.05),SLE患者血浆IL-31水平与IL-6无明显相关性(P>0.05);抗Sm抗体阳性组和阴性组、抗Ro-52抗体阳性组和阴性组、抗RNP抗体阳性组和阴性组各组间的IL-31水平差异均有统计学意义(P<0.05)。结论IL-31可能参与SLE发病,患者外周血IL-31和自身抗体水平检测有助于SLE的诊断。
Objective To detect the plasma interleukin(IL)-31 and IL-6 levels in the patients with systemic lupus erythematosus(SLE)and to investigate the relationship between IL-31 with autoantibodies.Methods Forty-two cases of SLE and 25 people undergoing healthy physical examination were selected.The plasma IL-31 level was measured by ELISA.The plasma IL-6 level was detected by electrochemiluminescence method.The ANA level was detected by the indirect immunofluorescence method.The autoantibodies[ds-DNA,anti-Sm antibody,anti-Sjogren′s A antibody,anti-Sjogren′s B antibody,anti-ribosome P antibody,anti-Sjogren′s Ro-52(anti-Ro-52)antibody,anti-histone antibody,anti-nucleosome antibody,anti-ribonucleoprotein(anti-RNP)antibody,topoisomerase I antibody,centromere antibody,anti-centromere B antibody]were detected by western blot.Results The levels of IL-31 and IL-6 in the SLE group were significantly higher than those in the healthy control group(P<0.05).No significant correlation was detected between plasma IL-31 level and IL-6 level in the patients with SLE.The IL-31 levels had statistical difference between the anti-Sm antibody positive group and negative group,between the anti-Ro-52 antibody positive group and negative group,between anti-RNP antibody positive group and negative group(P<0.05).Conclusion IL-31 may participate in mediating SLE pathogenesis.The peripheral blood IL-31 and autoantibodies detection is conducive to diagnose SLE.
作者
石渝
何应中
钱媛
伍小雪
苟晓琴
黄俊琼
SHI Yu;HE Yingzhong;QIAN Yuan;WU Xiaoxue;GOU Xiaoqin;HUANG Junqiong(Department of Clinical Laboratory,Affiliated Hospital of Zunyi Medical College,Zunyi,Guizhou 563000,China)
出处
《检验医学与临床》
CAS
2019年第22期3233-3235,3239,共4页
Laboratory Medicine and Clinic
基金
国家自然科学基金项目（81260266）
