摘要
目的:研究Th17相关的4种细胞因子IL-17,IL-21,IL-22,IL-23在系统性红斑狼疮(SLE)患者与健康人群中血清含量的差别和SLE患者血清中这4种细胞因子的含量与实验室检查指标的关联。方法:收集65例SLE患者和36例健康体检者的血清,用酶联免疫吸附试验(ELISA)检测2组样本血清中Th17细胞相关的4种细胞因子IL-17,IL-21,IL-22,IL-23的含量,将SLE患者与健康人群血清中4种细胞因子的含量进行对比,观察两者之间的差异性;将SLE患者血清中这4种细胞因子的含量与实验室指标进行比较,观察其相关性。采用SPSS19.0进行统计分析。结果:SLE患者血清中IL-17和IL-21的含量显著高于正常对照组(P<0.01),SLE患者血清中IL-22和IL-23的水平相对于正常组差异无统计学意义;65例SLE患者血清抗-dsDNA抗体,抗核小体,抗组蛋白阳性组与阴性组比较,阳性组IL-17含量均显著增高(P<0.05);相反,抗-Sm抗体阳性组与阴性组比较,阳性组IL-17含量显著降低(P<0.05);65例SLE患者血清中的IL-17,IL-21含量与C3含量之间均有显著关联(P<0.05),而这4种细胞因子与IgG含量无关联。结论:SLE患者血清里Th17相关细胞因子IL-17,IL-21的升高可能促进了疾病的发生与发展;通过对4种细胞因子含量与实验室指标的关联分析,说明IL-17可能促进了SLE患者体内抗-dsDNA抗体,抗核小体,抗组蛋白等的分泌,同时也说明IL-17含量的高低可能对SLE患者疾病的严重程度有一定的提示作用。
Objective：To study the serum expression differences of the four kinds of cytokines related to Th17 cells including IL-17,IL-21,IL-22 and IL-23 between the patients with systemic lupus erythematosus（SLE）and the healthy people,and analyze the association between the serum levels of these four cytokines in SLE patient and the laboratory test indicators.Method：The serum of 65 SLE patients and 36 health checkers were collected and the levels of the four cytokines IL-17,IL-21,IL-22 and IL-23 were detected using enzyme linked immunosorbent assay（ELISA）.The serum levels of these four cytokines in SLE patients and the healthy group were compared.The serum levels of these four cytokines and other laboratory indicators in SLE patient were compared,and the correlation was analyzed.Statistical analysis was performed using SPSS19.0.Result：The levels of IL-17 and IL-21 in SLE patients were significantly higher than those in the control group（P〈0.01）.But the levels of IL-22 and IL-23 in SLE patients were not significantly different from those in the control group.Comparing positive group of anti dsDNA antibody,anti-nucleosome antibody and anti-histone antibody in the 65 SLE patients with the negative group,the content of IL-17 in the positive group was significantly higher（P〈0.05）.On the contrary,comparing the anti-Sm antibody positive group with the negative group,the content of IL-17 in the positive group was significantly reduced（P〈0.05）.The contents of IL-17,IL-21 and C3 in the 65 SLE patients were significantly correlated（P〈0.05）,but there was no correlation between these four cytokines and IgG content.Conclusion：The increase of serumIL-17 and IL-21 of SLE patients may contribute to the occurrence and development of the disease.The correlation analysis of four cytokine factors and laboratory indexes showed that IL-17 may promote the secretion of anti-dsDNA antibody,anti-nucleosome antibody and anti-histone antibody in SLE patients,and the level of IL-17 may have a certain effect on th
出处
《临床血液学杂志（输血与检验）》
CAS
2017年第6期913-916,共4页
Journal of Clinical Hematology(Blood Transfusion & Laboratory Medicine)
