摘要
目的研究外周5-羟色胺(5-HT)在1型糖尿病大鼠进程中的变化,并探究其与结肠动力障碍之间的关系。方法使用链脲佐菌素(STZ)诱导大鼠1型糖尿病,通过免疫荧光与高效液相色谱质谱联用法(HPLS-MS)分别检测糖尿病动物结肠与外周血中5-HT的含量变化;通过Western印迹法检测糖尿病大鼠结肠黏膜层中色氨酸羟化酶1(TPH1)的表达改变;通过检测洋红运输时间考察结肠动力学的改变,观察外源性5-HT(10 mg/kg,腹腔注射)对糖尿病大鼠体质量、血糖以及结肠动力障碍的影响。结果与非糖尿病组相比,STZ诱导的1型糖尿病模型大鼠结肠黏膜层5-HT阳性表达细胞在模型建立后1周(P=0.000 3)、2周(P=0.002)和4周(P<0.000 1)均有明显减少;血清中的5-HT浓度在模型建立后3周(P=0.002 1)和4周(P=0.000 9)时明显降低,但黏膜层TPH1表达水平没有明显改变;外源性给予5-HT不影响糖尿病大鼠体质量下降和血糖升高,但可以缓解糖尿病大鼠的结肠动力障碍(P=0.005 0)。结论1型糖尿病动物外周5-HT水平缺乏可能与糖尿病动物结肠动力障碍有关。
Objective To investigate the association of peripheral 5-hydroxytryptamine(5-HT)with colonic dysmotility in type 1 diabetic rats.Methods The type 1 diabetes was induced by injection of streptozotocin(STZ)in rats.The levels of 5-HT in colon tissue and peripheral blood of diabetic rats were examined by immunofluorescence and HPLS-MS,respectively.The expression of tryptophan hydroxylase 1(TPH1)in the mucosa of diabetic rats was detected by Western blot.The colonic motility was evaluated by measuring the transit of carmine red.The effects of exogenous 5-HT(10 mg/kg,i.p.)on body weight,blood glucose and colonic motility were measured in diabetic rats.Results Compared with non-diabetic rats,in STZ-induced type 1 diabetic rats,the 5-HT-immunoreactive cells in mucosa of colon was significantly less at 1 week(P=0.000 3),2 week(P=0.002)and 4 week(P<0.000 1)after induction of diabetes.The 5-HT level in serum was significantly decreased at 3 week(P=0.002 1)and 4 week(P=0.000 9)after establishment of diabetes.However,the expression of TPH1 in mucosa was not significantly changed.The exogenous 5-HT improved colonic dysmotility(P=0.005 0),while it did not affect the loss of body weight and increase of blood glucose in type 1 diabetic rats.Conclusion The impairment of peripheral 5-HT is associated with colonic dysmotility in type 1 diabetic rats.
作者
成颖莹
王娟
戎伟芳
张国花
CHENG Ying-ying;WANG Juan;RONG Wei-fang;ZHANG Guo-hua(Dept.of Anatomy and Physiology,Basic Medical College,Shanghai Jiao Tong University,Shanghai 200025,China)
出处
《同济大学学报（医学版）》
CAS
2019年第5期548-553,共6页
Journal of Tongji University(Medical Science)
基金
国家自然基金面上项目(81770533)
