摘要
目的:分析颞下颌关节骨关节炎中软骨下骨骨质改建情况,检测Sox9、MMP-13、VEGF的表达并探讨它们对软骨下骨骨质改建的作用机制。方法:在兔关节盘的后外侧制备直径为2.5 mm全层穿孔,micro-CT扫描并计算髁突顶部软骨下骨的BV/TV,BS/BV,Tb.Th,Tb.Sp和Tb.N,HE染色及免疫组织化学方法检测髁突中Sox9、MMP-13、VEGF的表达和定位并进行半定量分析。结果:实验组表现为典型的骨关节炎组织病理学特征。实验组软骨下骨BV/TV、Tb.Th和Tb.N明显下降(P<0.05),BS/BV和Tb.Sp明显上升(P<0.05)。实验组软骨下骨中Sox9、MMP-13、VEGF的表达量明显高于假手术组(P<0.05)。结论:软骨下骨改建是TMJOA的一个重要特征,Sox9、MMP-13、VEGF对软骨下骨骨质改建具有重要意义。
Objective:To analyze the reconstruction of subchondral bone of TMJOA,to investigate the expression of Sox9,MMP-13,and VEGF,and to explore the mechanism of their effects on subchondral bone reconstruction.Methods:A full-thickness perforation in the diameter of 2.5 mm was made on the posterolateral side of the articular disc.BV/TV,BS/BV,Tb.Th,Tb.Sp,and Tb.N of subchondral bone were calculated by micro-CT.HE staining and immunohistochemical staining was carried out to analyze the expression and localization of Sox9,MMP-13,and VEGF,and semi-quantitative analysis was carried out.Results:Experimental group(EG)showed typical histopathological features of OA.BV/TV,Tb.Th,and Tb.N of EG were significantly decreased(P<0.05),and BS/BV and Tb.Sp of EG were significantly increased(P<0.05).The expression of Sox9,MMP-13,and VEGF in the subchondral bone of EG were significantly higher than those of sham operation group(SG)(P<0.05).Conclusion:The reconstruction of subchondral bone is an important feature of TMJOA.Sox9,MMP-13,and VEGF play an important role in bone remodeling.
作者
肖艳菊
吴国民
张瑶瑶
陈楷
王祥伸
XIAO Yanju;WU Guomin;ZHANG Yaoyao;CHEN Kai;WANG Xiangshen(Department of Oral and Maxillofacial Surgery,Stomatology Hospital,Jilin University,Changchun 130021,China;Key Laboratory of Tooth Development and Bone Remodeling and Regeneration,Jilin Province,Changchun 130021,China)
出处
《口腔医学研究》
CAS
北大核心
2019年第10期994-1000,共7页
Journal of Oral Science Research
基金
国家自然科学青年基金(编号:81400532)
关键词
颞下颌关节
骨关节炎
关节盘穿孔
软骨下骨
temporomandibular joint
osteoarthritis
perforation of articular disc
subchondral bone
