摘要
目的:探究血管生成相关因子及润滑相关因子在颞下颌关节骨关节炎滑膜中的表达。方法:分离人颞下颌关节滑膜组织,组织块法进行滑膜成纤维细胞的原代及传代培养。流式细胞仪检测细胞中CD34及CD44的表达,Western blotting和荧光定量PCR分别用于检测VEGF和TSP1蛋白,PRG4,HAS2和HAS3基因的表达。结果:骨关节炎滑膜细胞中VEGF、CD34和HAS3的表达显著高于对照组,但TSP1蛋白、PRG4和HAS2基因的表达低于对照组。结论:在颞下颌关节骨关节炎滑膜成纤维细胞中,血管生成相关因子高表达,而提高润滑作用的基因表达降低,该现象可能与该病的疾病发展有关。
Objective：To investigate the expression of angiogenic-associated factors and lubricating-associated genes in the synovial fibroblasts of osteoarthritis（OA）.Methods：Synovial tissues were collected from the temporomandibular joint of OA patients,and synovial fibroblasts were separated and cultured by tissue culture method.Flow cytometric analysis was used to examine the expression of CD34 and CD44.The protein expressions of VEGF and TSP1 were detected by western blotting.Real-time PCR was used to examined the gene expression of PRG4,HAS2 and HAS3.Results：The expressions of VEGF,CD34 and HAS3 were significantly increased in the synovial cells of OA,but TSP1,PRG4 and HAS2 expression were decreased.Conclusion：Proangiogenic factors were increased,and the lubricating-associated factors were decreased in the synovial cells of temporomandibular joint OA,suggesting that the synovium might play a beneficial role in the pathogenesis of OA by regulating the expression of these factors.
作者
郭慧琳
房维
邓末宏
龙星
GUO Hui --lin;FANG Wei;DENG Mo --hong;LONG Xing(Department of 2nd Oral and Maxillofacial Surgery, School of Stomatology , Wuhan University, Wuhan 430079, China;The State Key Laboratory Breeding Base of Basic Science of Stomatology and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology , Wuhan University, Wuhan 430079, China)
出处
《口腔医学研究》
CAS
北大核心
2018年第3期270-273,共4页
Journal of Oral Science Research
基金
国家自然科学基金(编号:81470761
81771100)
武汉大学青年教师(编号:2042017kf0140)
