摘要
目的:探讨光动力疗法(PDT)联合Er:YAG激光对大鼠口腔内变异链球菌的杀菌性及对牙体硬组织抗酸性的影响。方法:将28只大鼠动物模型随机分为7组进行处理,通过分光光度法测定OD值,反映各组变形链球菌的生长变化情况;经0.1 mmol/L乳酸液浸泡24 h后检测钙磷溶出量;采用数字显微硬度仪进行乳酸处理前后的硬度测试;扫描电镜下观察各组牙齿表面形态、清洁度及牙本质小管开口等情况。结果:与空白对照组比较,各实验组变形链球菌的生长出现显著下降,经乳酸处理后均有不同程度的钙、磷溶出,表面平均显微硬度值也均有降低;扫描电镜观察PDT联合Er:YAG激光组表面未发现玷污层,可见牙本质小管出现明显的熔融封闭。结论:PDT联合Er:YAG激光处理龋齿,既能高效杀灭致龋菌又能明显封闭牙本质小管,提升牙体表面的抗酸性,有效预防龋齿的发生。
Objective:To investigate the bactericidal effect of photodynamic therapy(PDT)combined with Er:YAG laser on dental caries in rats and the acid resistance of dental hard tissue.Methods:28 rats were randomly divided into 7 groups.The germicidal efficacy of each group was observed by spectrophotometer to determine the optical density(OD)value.The dissolution of calcium and phosphorus was detected after being soaked in 0.1 mmol/L lactic acid solution for 24 hours.The hardness of each group was tested by digital microhardness tester before and after lactic acid treatment.The tooth surface morphology,cleanliness,and dentinal tubules were observed by scanning electron microscope(SEM).Results:Compared with negative control group,the growth of bacteria of each group was significantly reduced.After lactic acid treatment,calcium and phosphorus were dissolved at different degrees,and the average microhardness of the surface was decreased.SEM observation showed that the surface of PDT combined with Er:YAG laser group was smooth and had obvious melting sealing effect on dentinal tubules.Conclusion:PDT combined with Er:YAG laser treatment of dental caries can not only kill cariogenic bacteria effectively,but also seal dentinal tubules significantly.It enhanced the acid resistance of tooth hard tissue and prevent the dental caries.
作者
张蕾
姚丽萍
孟磊
ZHANG Lei;YAO Liping;MENG Lei(Stomatology Hospital of Yantai,Yantai 264008,China)
出处
《口腔医学研究》
CAS
北大核心
2019年第10期961-965,共5页
Journal of Oral Science Research
基金
烟台市科技发展计划项目(编号:020YLPKYF)
