摘要
目的探讨益气活血散结方对肝纤维化大鼠转化生长因子-β1(TGF-β1)及血管内皮生长因子(VEGF)的影响。方法选择健康SD大鼠56只,随机分为正常组(8只)、模型组(12只)、秋水仙碱组(9只)、益气活血散结方低剂量组(9只)、益气活血散结方中剂量组(9只)、益气活血散结方高剂量组(9只)。正常组皮下注射橄榄油,其他5组注射40%的CCl 4和橄榄油造模,连续6周。自造模之日起,正常组和模型组给予生理盐水灌胃,秋水仙碱组给予秋水仙碱0.1 mg/(kg·d)灌胃,益气活血散结方低、中、高剂量组分别给予0.55 g/(kg·d)、1.1 g/(kg·d)、2.2 g/(kg·d)的益气活血散结方灌胃,连续10周。灌胃结束后,取各组大鼠血液,采用ELISA法检测TGF-β1及VEGF水平,然后取肝脏,HE染色及胶原纤维染色后观察各组大鼠肝脏组织病变情况。结果模型组大鼠血清TGF-β1及VEGF水平均明显高于其他5组(P均<0.05);益气活血散结方低、中、高剂量组随着给药剂量的升高,TGF-β1及VEGF水平均逐渐降低(P均<0.05)。模型组肝纤维化程度严重;秋水仙碱组及益气活血散结方各组肝纤维化程度均明显轻于模型组。结论益气活血散结方可抑制TGF-β1及VEGF的表达,具有抗肝纤维化作用。
Objective It is to investigate the effects of Yiqi Huoxue Sanjie decoction on transforming growth factor-β1(TGF-β1)and vascular endothelial growth factor(VEGF)in rats with hepatic fibrosis.Methods A total of 56 healthy SD rats were randomly divided into normal group(8 rats),model group(12 rats),colchicine group(9 rats),low-dose of Yiqi Huoxue Sanjie group(9 rats),medium-dose of Yiqi Huoxue Sanjie group(9 rats),high-dose of Yiqi Huoxue Sanjie group(9 rats).The normal group was injected with olive oil subcutaneously,and the other 5 groups were injected with 40%CCl 4 and olive oil for model establishing,all the groups were treated for 6 weeks.From the day of modeling,the normal group and model group were given normal saline by gastric lavage,colchicine group was given colchicine 0.1 mg/(kg·d)by gastric lavage,and low,medium and high dose of Yiqi Huoxue Sanjie groups were given Yiqi Huoxue Sanjie decoction 0.55 g/(kg·d),1.1 g/(kg·d)and 2.2 g/(kg·d)respectively by gastric lavage,all the treatment lasted for 10 weeks.At the end of the gavage,the blood of each group was taken to detect the levels of TGF-β1 and VEGF by ELISA,their liver were taken to observe the lesions of liver tissue in each group after HE staining and collagen fiber staining.Results The levels of serum TGF-β1 and VEGF in the model group were significantly higher than those in the other 5 groups(P<0.05).With the increase of the dose,the levels of TGF-β1 and VEGF gradually decreased in low,medium and high dose of Yiqi Huoxue Sanjie groups(P<0.05).The degree of liver fibrosis was severe in the model group,but that of liver fibrosis in the colchicine group and the Yiqihuoxuejiejie groups were significantly less serious than those in the model group.Conclusion Yiqi Huoxue Sanjie decoction can inhibit the expression of TGF-β1 and VEGF and has anti-fibrosis effect.
作者
孙兆
杨如意
曹昌霞
赵文玲
徐海珍
刘永萍
SUN Zhao;YANG Ruyi;CAO Changxia;ZHAO Wenling;XU Haizhen;LIU Yongping(Affiliated Hospital of Qinghai University,Xining 810001,Qinghai,China)
出处
《现代中西医结合杂志》
CAS
2019年第27期2965-2968,共4页
Modern Journal of Integrated Traditional Chinese and Western Medicine
基金
青海大学2017年度中青年科研基金项目(2017-QYY-12)
关键词
益气活血散结方
肝纤维化
转化生长因子-Β1
血管内皮生长因子
Yiqi Huoxue Sanjie decoctoin
liver fibrosis
transforming growth factor-β1
vascular endothelial growth factor
