摘要
目的通过网络药理学技术对丹酚酸B(SAB)治疗增生性瘢痕(HS)进行多靶点预测分析,探讨其可能作用机制。方法联用GeneCards、SEA、SIB、ChEMBL数据库获取SAB作用靶点,通过Genecards及DisGeNET数据库预测HS相关靶点,筛选出SAB治疗HS的潜在靶点并运用Cytoscape软件进行可视化处理,得到“成分-靶点-疾病”网络;利用STRING数据库构建PPI网络并运用Cytoscape软件进行可视化处理;利用Cytoscape软件中ClueGO插件对靶点进行GO细胞组成、分子功能、生物进程分析;运用R软件3.5.3中ClusterProfile 3.8工具包对潜在靶点进行KEGG信号通路富集分析。结果筛选出42个潜在靶点并通过构建PPI网络反映靶点间的相互关系。GO和KEGG分析结果表明,这些靶点涉及多方面的分子功能及多种生物过程并通过调控IL-17、TNF、HIF-1信号通路等128条通路来发挥抗瘢痕增生的作用。结论本研究反映了SAB经多靶点、多通路协同作用抑制HS的作用特点,初步阐明其潜在作用机制,为后续研究开展提供新思路。
Objective To investigate the possible mechanism of Salvianolic acid B(SAB)in the treatment of hypertrophic scar(HS)by multi-target prediction and analysis with network pharmacology.Methods The targets of SAB were obtained from GeneCards,SEA,SIB and ChEMBL database and the targets of HS were predicted by Genecards database and DisGeNET database.The potential targets of SAB for HS were selected and visualized by Cytoscape software.Subsequently,"component-target-disease"network was established.The PPI network was constructed by STRING database and visualized by Cytoscape software.Then,ClueGO plug-in in Cytoscape software was used to analyze GO cell composition,molecular function and biological process.Cluster Profile 3.8 package in R software 3.5.3 was used to enrich KEGG signaling pathways for potential targets.Results 42 potential targets were screened and PPI network was constructed to reveal the relationship of the targets.GO and KEGG analysis showed that these targets involved various molecular functions and biological processes,and inhibited HS by regulating 128 pathways such as IL-17 signaling pathway,TNF signaling pathway and HIF-1 signaling pathway.Conclusion This study shows the functional characteristics of SAB inhibiting HS by multi-target and multi-pathway synergetic action,and elucidates its possible mechanism,which provides ideas for further research.
作者
许小琪
杨松林
王军
赖建辉
郭思旖
王芳
陈求芳
时军
XU Xiaoqi;YANG Songlin;WANG Jun;LAI Jianhui;GUO Siyi;WANG Fang;CHEN Qiufang;SHI Jun(School of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou 510006,China;Guangzhou Annuo Technology Co.,Ltd,Guangzhou 510530,China;Guangdong Engineering&Technology Research Center of Precise Drug Delivery System,Guangzhou 510006,China)
出处
《广东药科大学学报》
CAS
2019年第4期523-528,共6页
Journal of Guangdong Pharmaceutical University
基金
广东省自然科学基金项目(2018A0303130234)
广东省高等学校优秀青年教师培养计划项目(YQ2015099)
关键词
丹酚酸B
增生性瘢痕
多靶点
Salvianolic acid B
hypertrophic scar
multi-target
