摘要
目的:观察丹酚酸B(Sal B)对人肺成纤维细胞转化生长因子β1(TGF-β1)/Smad信号通路相关蛋白表达的影响,探讨其抑制TGF-β1活化肺成纤维细胞作用的信号机制。方法:体外培养人胚肺成纤维细胞MRC-5,随机分为对照组(未加入TGF-β1或Sal B)、Sal B(10μmol·L-1)组、TGF-β1(10μg·L-1)组和TGF-β1(10μg·L-1)+Sal B(10μmol·L-1)组。Western blotting法检测各组细胞内p-Smad2、p-Smad3、TGF-βⅠ型受体(TβRⅠ)和Smad7蛋白表达。结果:与对照组比较,TGF-β1组细胞内p-Smad2、p-Smad3和TβRⅠ蛋白表达水平均明显升高(P<0.05),Smad7蛋白表达水平明显下降(P<0.05);与对照组比较,Sal B组细胞内p-Smad2、p-Smad3、TβRⅠ和Smad7蛋白表达水平无明显变化(P>0.05);与TGF-β1组比较,TGF-β1+Sal B组细胞内p-Smad2、p-Smad3和TβRⅠ蛋白表达水平均下降(P<0.05),Smad7蛋白表达水平升高(P<0.05)。结论:Sal B能够通过抑制肺成纤维细胞TGF-β1/Smad信号通路,从而发挥其抑制TGF-β1活化肺成纤维细胞的作用。
Objective To investigate the influence of salvianolic acid B on the expressions of TGF-β1/Smad signaling pathway related proteins in human lung fibroblasts,and to explore the mechanism of the inhibitory effect of Sal B on TGF-β1-induced lung fibroblast activation.Methods The human embryonic lung fibroblasts（MRC-5）were cultured in vitro and randomly divided into control group（the cells were cultured with DMEM without TGF-β1or Sal B）,Sal B group（the cells were cultured with 10μmol·L-1 Sal B）,TGF-β1group（the cells were cultured with 10μg·L^-1 TGF-β1）,and TGF-β1（10μg·L^-1）＋ Sal B（10μmol·L^-1）group.The protein levels of p-Smad2,p-Smad3,TβRⅠ,and Smad7in the fibroblasts in various groups were detected by Western blotting method.Results Compared with control group,the expression levels of p-Smad2,p-Smad3,and TβRⅠproteins in TGF-β1group were significantly increased（P〈0.05）,and the expression level of Smad7protein was decreased（P〈0.05）.Compared with control group,the expression levels of p-Smad2,p-Smad3,TβRⅠ,and Smad7proteins in lung fibroblasts in Sal B group had no significant change（P〉0.05）.Compared with TGF-β1group,the expression levels of p-Smad2,p-Smad3,and TβRⅠin TGF-β1＋ Sal B group were descended（P〈0.05）,and the expression level of Smad7was increased（P〈0.05）.Conclusion Sal B could suppress the TGF-β/Smad signaling pathway in lung fibroblasts and to inhibit the TGF-β1-induced lung fibroblast activation.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2014年第4期705-709,共5页
Journal of Jilin University：Medicine Edition
基金
国家自然科学基金资助课题(81100042)
广东省卫生厅医学科研基金项目资助课题(A2012498)
广东省广州市卫生局医药卫生科技一般引导项目资助课题(20121A011044)
