摘要
生长素反应因子(auxin response factors,ARF)是一类重要的调控植物生长发育的转录因子,参与生长素响应基因的表达和生长素信号转导途径等多种发育过程。本研究采用比较基因组学方法对大豆、百脉根、蒺藜苜蓿的生长素反应因子(ARF)家族的理化性质、系统发育关系、结构特性、多倍化与基因家族的关联性,以及重复基因进行分析。3个物种中共鉴定出129个ARF家族基因,理化性质差异较大;系统发育分析显示全部基因被分为4个亚族,亲缘关系较近的基因表现出相似的结构和基序组成,选择压力分析发现有26个位点受到正选择;另外,大豆基因组内ARF同源基因对的数量大于其它物种,表明全基因组加倍导致产生大量的重复基因,百脉根ARF同源基因对数量少于蒺藜苜蓿,证明了加倍事件伴随着重复基因的差异性丢失。这些结果将为豆科或其它物种ARF基因的深入研究提供一定的参考依据。
Auxin response factors(ARF)are a kind of important transcription factors in regulating plant growth and development,which are involved in various plant development processes,such as the expression of auxin response genes and the signal transduction pathway of auxin.Based on comparative genomics analysis,the physicochemical properties,structural characteristics,phylogenetic relationship,the relation between polyploidy and family genes,and the duplicate genes of ARF family in soybean,Lotus corniculatus and Medicago truncatula were analyzed.129 ARF family genes with significant difference of physicochemical properties were identified in the three species.Phylogenetic analysis showed that all the genes were clustered into 4 subgroups,and the genes with closer genetic relationship presented similar structures and motif compositions.The selection pressure analysis showed that 26 loci were positively selected.In addition,the number of ARF homologous gene pairs in the soybean genome was larger than that of other species,which indicated that a large number of duplicate genes were produced caused by whole genome duplication(WGD).Meanwhile,the number of ARF homologous gene pairs in Lotus corniculatus was less than that of Medicago truncatula,which confirmed that the reduplication incident was missing with the difference of duplicate genes.These results would provide some reference for further study of ARF genes in legume or other species.
作者
刘润燕
聂仁清
孙仔妹
张岚
Liu Runyan;Nie Renqing;Sun Zimei;Zhang Lan(School of Life Sciences,North China University of Science and Technology,Tangshan063000)
出处
《分子植物育种》
CAS
CSCD
北大核心
2019年第14期4529-4536,共8页
Molecular Plant Breeding
基金
华北理工大学大学生创新项目(X2017011)资助
