摘要
运用生物信息学方法对棉花GhARF3基因序列进行BLAST分析、结构域分析、进化树分析、编码蛋白质的理化性质分析、疏水性分析、二硫键分析、磷酸化位点分析、二级结构以及三级结构的预测、亚细胞定位和蛋白质功能分类预测等分析。分析结果表明GhARF3具有ARF类转录因子的特性,可能参与棉花纤维发育的调控。为进一步研究GhARF3基因功能提供理论指导。
Auxin response factor （ARF） is an important transcription factor for auxin signal transduction pathway. In order to identify the function of cotton auxin response factor GhARF3, some bioinformatics methods were used to analyze GhARF3 gene sequence, including BLAST analysis, domain analysis, phylogenetic tree analysis, the physical and chemical properties analysis of protein, hydrophobicity analysis, disulfide bonds analysis, phosphorylation site analysis, secondary structure and tertiary structure prediction, and subcellular localization and protein functional classification analysis. The results show that the GhA RF3 has the characteristics of transcription factor. The GhA RF3 may be involved in the regulation of the development of cotton fiber. This might provide some reference for studying the GhARF3 gene function.
出处
《热带农业科学》
2009年第7期5-12,共8页
Chinese Journal of Tropical Agriculture
基金
国家重点基础研究发展规划(973计划)项目(No.2004CB117307)
