摘要
目的:建立同时测定市售茵栀黄颗粒中新绿原酸、绿原酸、京尼平苷、1,3-二咖啡酰奎宁酸、木犀草苷、3,4-二咖啡酰奎宁酸、3,5-二咖啡酰奎宁酸、4,5-二咖啡酰奎宁酸、黄芩苷、滨蒿内酯、汉黄芩素、黄芩素、汉黄芩苷及千层纸素A共14个化学成分含量的高效液相色谱方法。方法:采用Diamonsil C18(200 mm×4.6 mm,5μm)色谱柱,以乙腈-0.1%甲酸水溶液为流动相,梯度洗脱,流速1.0mL·min^-1,柱温30℃,检测波长325 nm,进样量20μL。结果:茵栀黄颗粒中上述14个化学成分均可实现较好的分离,在一定的线性范围内呈良好的线性关系(r≥0.999 0)、精密度(RSD<2.5%)、稳定性(RSD<2.5%)和重复性(RSD<2.5%)良好,平均回收率(n=9)在95.0%~105.0%范围内,RSD在0.8%~3.1%范围内。8批样品中上述14个成分的含量范围分别为0.535~0.653、2.228~2.717、5.171~5.833、0.216~0.274、0.618~0.827、0.323~0.452、0.163~0.386、0.511~0.685、68.515~77.164、0.013~0.018、0.206~1.356、0.797~1.915、0.174~0.786、0.035~0.136 mg·g^-1。结论:该方法可用于茵栀黄颗粒的质量控制与评价。
Objective:To establish an HPLC method for simultaneous determination of neochlorogenic acid,chlorogenic acid,geniposide,1,3-dicaffeoylquinic acid,luteoloside,3,4-dicaffeoylquinic acid,3,5-dicaffeoylquinic acid,4,5-dicaffeoylquinic acid,baicalin,scoparone,wogonin,baicalein,wogonoside and oroxylin A in Yinzhihuang granules bought from market.Methods:High-performance liquid chromatography was carried out on a Diamonsil C18(200 mm × 4.6 mm,5 μm)with the mobile phase of acetonitrile(A)-0.1%formic acid water solution(B)at a flow rate of 1.0 mL·min^-1,a column temperature of 30 ℃,a detection wavelength of325 nm and the sample size of 20 μL by using gradient elution.Results:Above-mentioned 14 compounds were separated well and showed an excellent linear relationship(r≥0.999 0)with high precision,stability and repeatability(RSD<2.5%).In addition,the average rate(n=9)of the 14 above-mentioned compounds was95.0%-05.0% with the RSD value of 0.8%-3.1%.The contents of the above-mentioned 14 compounds of 8 groups were 0.535-0.653,2.228-2.717,5.171-5.833,0.216-0.274,0.618-0.827,0.323-0.452,0.163-0.386,0.5 11-0.685,68.515-77.164,0.013-0.018,0.206^-1.356,0.797^-1.915,0.174-0.786,0.035-0.136 mg·g^-1,respectively.Conclusion:This method can be used as an efficient method for quality control and evaluation of Yinzhihuang granules.
作者
张玉姗
刘谢英
姚新成
罗莉
ZHANG Yu-shan;LIU Xie-ying;YAO Xin-cheng;LUO Li(School of Pharmacy,Shihezi University Shihezi 832000,China;Key Laboratory of Xinjiang PhytomedicineResource and Utilization,Ministry of Education,Shihezi University,Shihezi 832000,China)
出处
《药物分析杂志》
CAS
CSCD
北大核心
2019年第7期1229-1238,共10页
Chinese Journal of Pharmaceutical Analysis
关键词
茵栀黄颗粒
茵陈
栀子
黄芩
金银花
新绿原酸
绿原酸
京尼平苷
二咖啡酰奎宁酸
木犀草苷
黄芩苷
滨蒿内酯
汉黄芩素
黄芩素
汉黄芩苷
千层纸素A
含量测定
高效液相色谱
Yinzhihuang granules
Artemisia capillaris Thunb.
Gardenia jasminoides Ellis
Scutellaria baicalensisGeorgi
Lonicera japonica Thunb.
neochlorogenic acid
chlorogenic acid
geniposide
dicaffeoylquinic acid
luteoloside
baicalin
scoparone
wogonin
baicalein
wogonoside
oroxylin A
content determination
HPLC
