摘要
对精细定位某一特定性状位点来说,高密度遗传图谱是一个非常实用的工具。本研究以水稻品种V20B/CPSLO17组合衍生的150份重组自交家系作为作图群体,利用特定位点扩增长度测序(SLAF-seq)技术,一种基于下一代测序技术进行大规模开发SNP和基因型分析的高通量新策略,开发SLAF标签和构建水稻高密度遗传图谱。我们共检测到67 017个高质量的SLAF标签,其中多态性SLAF有15 853个,符合构建高质量遗传图谱使用要求的多态性SLAF标签有8 657个。最终成功构建了一个包含12个连锁群,8 602个上图标记,总图距为2 508.65 c M,标记间平均距离为0.29 c M的高密度遗传图谱,该图谱可用于重要农艺性状QTL定位。
High-density genetic m ap is a useful and valuable tool for fine mapping locus controlling a specific trait. In this study, a mapping population of 150 lines(recombinant inbred lines) derived from a cross between rice varieties V20 B and CPSLO17, and the specific locus amplified fragment sequencing(SLAF-seq) technology, a new high-throughput strategy for large-scale SNP discovery and genotyping based on next generation sequencing(NGS), were employed to develop SLAF markers and construct a high-density genetic map of rice(Oryza sativa L.).We firstly detected a total of 67 017 high-quality SLAF markers, of which 15 853 SLAFs were polymorphic and8 657 of the polymorphic markers met the requirements for use in the construction of a high-quality genetic map.The final genetic map included 8 602 markers on the 12 linkage groups(LGs) and was 2 508.65 c M in length with an average distance of 0.29 c M between adjacent markers. The above results showed that the high-density map was successfully constructed by SLAF-seq technology could be used to mapping important agronomic traits in rice.
出处
《分子植物育种》
CAS
CSCD
北大核心
2016年第8期2127-2132,共6页
Molecular Plant Breeding
基金
贵州省联合基金项目(黔科合LH字(2014)7689号)
贵州省农科院专项(黔农科院院专项(2010)004号)
贵州省科技计划项目(黔科合重大专项字(2013)6023号)
贵州省重大专项(黔科合重大专项字(2012)6005号)
贵州省工程中心续建项目(黔科合农G字(2012)4010)共同资助
关键词
水稻
遗传图谱
特定位点扩增片段
作图群体
Oryza sativa L.
Genetic map
Specific locus amplified fragment
Mapping population
