摘要
研究甲基维生素B12 对脱髓鞘的修复作用。以实验性自身免疫性脑脊髓炎作为多发性硬化的动物模型。用豚鼠脊髓匀浆和完全弗氏佐剂诱导Lewis大鼠发生实验性自身免疫性脑脊髓炎 (experimentalauto immuneen cephalomyelitis,EAE) ,给予不同剂量的甲基维生素B12 ,观察体重变化、神经系统评分和组织学检查。注射甲基维生素B12 (2 6 6 μg/kg ,5 32 μg/kg)增加大鼠的缓解比例 ,由 35 .7%增至 6 0 %。降低死亡率 ,由 5 2 .3%降为 16 .70 % ,减少体重 (g)下降量 ,由 18.9± 15 .6降到 4 .8± 9.5。组织学检查证实甲基维生素B12 有促进髓鞘修复的作用。提示 ,较大剂量甲基维生素B12 对多发性硬化模型大鼠有确切良好作用。
The study was designed to evaluate the effectiveness of methyl vitamin B 12 on experimental multiple sclerosis(MS), which causes a widespread demyelination in central nervous system. Experimental auto immune encephalomyelitis (Multiple Sclerosis model) was induced by injection of homogenates of guinea pig spinal cord which was mixed with Freund's complete adjuvant. Observations of body weight, neurological score and histopathological changes were carried out in 3 groups of different doses of methyl vitamin B 12 . The results showed:Injection of methyl vitamin B 12 (266 μg/kg, 532 μg/kg) increased the remission rate, from 35.7 % to 60%; decreased the mortality, from 52.3% to 16.70% and decreased the loss of body weight (g), from 18.9 ±15.6 to 4.8±9.5. Histopathological examination also supported the positive effect of methyl vitamin B 12 on repairing myelin.It can be concluded that large dose of methyl vitamin B 12 have beneficial effects on EAE rats and it may help in treatment or auxiliary treatment of MS.
