摘要
目的 :介绍一种新的简便可靠的实验性变态反应性脑脊髓炎大鼠模型制备方法。方法 :用自制完全福氏佐剂 +豚鼠脊髓生理盐水匀浆制备成免疫抗原 ,给Wistar大鼠四足垫内注射。结果 :在接种后 1 2～ 1 8天 ,大鼠出现双后肢瘫 ,脊髓横切片HE染色可见血管周围袖套样炎性细胞浸润和前角运动神经元肿胀变性。结论 :本方法操作简单 ,可重复性强 ,适于广泛推广。
Objective: To establish a reliable and simple method for establishing the experimental allergic encephalomyelitis(EAE) in rats. Methods: Immune antigen was made from both self-made complete Freund adjuvant and spinal cord homogenate of guinea pig, and were injected into rats' paw-pads. Results: On day 12～18, rats were paralyzed on its 2 hind limbs, the coronal sections of lumbosacral enlargement of spinal cord with HE stainning showed that there were dilated capillaries, perivascular oversleeve-like inflammatory infilltration and anterior-horn motor-neuron hydropic degeneration. Conclusion: The method is easy to manipulate and reproduce, and is suitable for wide application.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2001年第1期77-81,共5页
Chinese Journal of Anatomy
