摘要
目的探讨淫羊藿苷是否通过调控转移相关蛋白2(MTA2)表达抑制人胃癌细胞系MGC803细胞增殖、迁移和侵袭及其作用机制。方法以不同浓度淫羊藿苷干预MGC803细胞,MTT法检测细胞活力;Transwell小室法和Western blot分别检测细胞迁移和侵袭及MMP-2、MMP-9和MTA2蛋白表达;将MTA2 siRNA转染至MGC803细胞后检测细胞活力、迁移和侵袭;将pc DNA-MTA2转染至MGC803细胞,联合淫羊藿苷处理,MTT法和Traswell小室法检测细胞活力、迁移和侵袭。结果不同浓度淫羊藿苷均能有效抑制MGC803细胞活力。以200μmol/L淫羊藿苷干预MGC803细胞后,细胞迁移和侵袭能力下降(P<0. 05),MMP-2、MMP-9、MTA2蛋白表达下调。敲减MTA2能够抑制细胞活力、迁移和侵袭。过表达MTA2可部分逆转淫羊藿苷对MGC803细胞活力、迁移和侵袭的抑制作用。结论淫羊藿苷能够通过调控MTA2、MMP-2和MMP-9蛋白表达,抑制胃癌细胞增殖、迁移和侵袭。
Objective To investigate the mechanism of icariin inhibiting proliferation, migration and invasion of MGC803 cells by regulating MTA2. Methods Cell viability of MGC803 cells after treatment with multiple concentrations of icariin was detected by MTT assay. Migration, invasion and the protein expression of MMP-2, MMP-9 and MTA2 in MGC803 cells were detected by Transwell and Western blot respectively. Transfection MTA2 siRNA into MGC803 cells, and detecting cell viability, migration and invasion. Cell viability, migration and invasion of MGC803 cells after transfection of pcDNA-MTA2 combined with icariin were detected by MTT assay and Traswell, respectively. Results Icariin of different concentrations effectively inhibited viability of MGC803 cells. Migration, invasion and the protein expression levels of MMP-2, MMP-9 and MTA2 of MGC803 cells after treatment with 200 μmol/L icariin were decreased. Knockdown MTA2 inhibited cell viability, migration and invasion. Over-expression of MTA2 partially reversed the inhibitory effect of icariin on the viability, migration and invasion of MGC803 cells. Conclusions Icariin can inhibit proliferation, migration and invasion of gastric cancer by down-regulating the expression of MTA2, MMP-2 and MMP-9.
作者
陈素华
马天江
张智慧
张磊
史磊
CHEN Su-hua;MA Tian-jiang;ZHANG Zhi-hui;ZHANG Lei;SHI Lei(Department of Oncology, Luohe Central Hospital, Luohe 462000, China)
出处
《基础医学与临床》
CSCD
2019年第11期1587-1591,共5页
Basic and Clinical Medicine
关键词
淫羊藿苷
转移相关蛋白2
胃癌
迁移
侵袭
icariin
metastasis-associated protein 2
gastric cancer
migration
invasion
